超声造影联合血清miR-29a、miR-126检测在肾癌诊断中的价值  被引量:1

Diagnostic efficiency of contrast-enhanced ultrasound combined with serum miR-29a and miR-126 in renal carcinoma

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作  者:李晓艳 吕亚玲 LI Xiaoyan;LYU Yaling(Department of Ultrasound,Luochuan County Hospital,Yan'an,Shaanxi 727400,China;Department of Urology,Xi'an Third People's Hospital,Xi'an,Shaanxi 710000,China)

机构地区:[1]陕西省延安市洛川县医院超声科,陕西延安727400 [2]陕西省西安市第三人民医院泌尿外科,陕西西安710000

出  处:《检验医学与临床》2024年第4期538-541,551,共5页Laboratory Medicine and Clinic

摘  要:目的观察超声造影联合血清微小RNA(miR)-29a、miR-126检测在肾癌诊断中的效能。方法选取陕西省延安市洛川县医院2019年2月至2022年2月86例肾癌患者作为肾癌组,另选取同期24例肾错构瘤患者作为良性组,所有患者均接受超声造影检查,采用实时荧光定量聚合酶链反应技术检测血清miR-29a、miR-126相对表达量,比较两组超声造影参数及血清miR-29a、miR-126相对表达量,采用受试者工作特征(ROC)曲线分析超声造影联合血清miR-29a、miR-126检测对肾癌的诊断价值;采用Pearson相关分析超声造影参数与血清miR-29a、miR-126的相关性。结果肾癌组PI高于良性组,TTP、ET均低于良性组,差异有统计学意义(P<0.05);肾癌组血清miR-29a相对表达量高于良性组,miR-126相对表达量低于良性组,差异有统计学意义(P<0.05);ROC曲线分析结果显示,PI、TTP、ET、血清miR-29a及miR-126联合检测诊断肾癌的曲线下面积(AUC)为0.931,高于5项指标单独检测的AUC(0.795、0.785、0.751、0.777、0.795,P<0.05)。肾癌患者超声造影参数PI与血清miR-29a相对表达量呈正相关(r=0.701,P<0.05),与miR-126相对表达量呈负相关(r=-0.735,P<0.05);TTP、ET均与miR-29a相对表达量呈负相关(r=-0.662、-0.635,P<0.05),与miR-126相对表达量呈正相关(r=0.682、0.647,P<0.05)。结论肾癌患者血清miR-29a水平升高、miR-126水平降低,超声造影联合血清miR-29a、miR-126检测对肾癌具有较高的诊断效能,可为肾癌的临床诊疗提供参考。Objective To observe the efficacy of contrast-enhanced ultrasound combined with serum microRNA(miR)-29a and miR-126 in the diagnosis of renal carcinoma.Methods A total of 86 patients with renal cancer from February 2019 to February 2022 in Luochuan County Hospital were selected as the renal cancer group,and 24 patients with renal hamartoma during the same period were selected as the benign group.All patients underwent contra-enhanced ultrasound examination.Real-time quantitative fluorescence polymerase chain reaction was used to detect the relative expression levels of serum miR-29a and miR-126.Contrast-enhanced ultrasound parameters and the relative expression levels of serum miR-29a and miR-126 were compared between the two groups.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of contrast-enhanced ultrasound combined with serum miR-29a and miR-126 in renal carcinoma.Pearson correlation was used to analyze the correlation between ultrasound parameters and serum miR-29a and miR-126.Results PI in renal cancer group was higher than that in benign group,TTP and ET were lower than those in benign group,the differences were statistically significant(P<0.05).The relative expression level of miR-29a in renal cancer group was higher than that in benign group,and the relative expression level of miR-126 was lower than that in benign group,with statistical significance(P<0.05).ROC curve analysis results showed that the area under the curve(AUC)of PI,TTP,ET,serum miR-29a and miR-126 combined detection in the diagnosis of renal carcinoma was 0.931,which was higher than that of the AUC detected by the five indexes alone(0.795,0.785,0.751,0.777,0.795,P<0.05).Contrast-enhanced ultrasound parameter PI correlated positively with the relative expression level of serum miR-29a(r=0.701,P<0.05),and correlated negatively with the relative expression level of miR-126(r=-0.735,P<0.05).TTP and ET correlated negatively with the relative expression level of miR-29a(r=-0.662,-0.635,P<0.05),and correlate

关 键 词:肾癌 超声造影 miR-29a MIR-126 实时荧光定量聚合酶链反应 

分 类 号:R737.1[医药卫生—肿瘤]

 

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