美沙拉嗪联合康复新液治疗对炎症性肠病患者BATF2表达的影响分析  

Effect of Mesalazine Combined with Kangfuxin Solution on the Expression of BATF2 in Patients with Inflammatory Bowel Disease

作　　者：陈元旺 丁斌[1] 薛国辉[2] 章欢[1] 胡颖 CHEN Yuanwang;DING Bin;XUE Guohui;ZHANG Huan;HU Ying(Jiujiang First People's Hospital Department of Gastroenterology,Jiujiang Jiangxi 332000,China;Jiujiang First People's Hospital Department of Laboratory Medicine,Jiujiang Jiangxi 332000,China;Jiujiang First People's Hospital Department of Pharmacy,Jiujiang Jiangxi 332000,China)

机构地区：[1]九江市第一人民医院消化内科,江西九江332000 [2]九江市第一人民医院检验科,江西九江332000 [3]九江市第一人民医院药剂科,江西九江332000

出　　处：《现代诊断与治疗》2023年第19期2845-2848,共4页Modern Diagnosis and Treatment

基　　金：江西省卫生健康委科技计划(项目编号202140243)。

摘　　要：目的探究美沙拉嗪联合康复新液治疗对炎症性肠病(Inflammatory bowel disease,IBD)患者肠粘膜碱性亮氨酸拉链ATF样转录因子2(Basic leucine zipper transcription factor ATF-like 2,BATF2)表达的影响。方法选取2019年5月至2022年8月我院收治的IBD患者76例作为观察组,其中溃疡性结肠炎(Ulcerative colitis,UC)和克罗恩病(Crohn's disease,CD)患者各38例。选取30例结肠镜检查未见结肠病变的健康体检人群作为正常对照组。收集观察组和对照组患者的新鲜肠粘膜组织,并收集美沙拉嗪联合康复新液治疗后的肠粘膜组织,荧光定量PCR测定BATF2 mRNA、白介素17(Interleukin,IL-17)mRNA和白介素10(Interleukin,IL-10)mRNA的表达水平。结果UC和CD患者肠粘膜组织中BATF2 mRNA均显著低于对照组,IL-17 mRNA显著高于对照组,而IL-10 mRNA显著低于对照组,差异有统计学意义(P<0.05)。UC患者中,BATF2 mRNA与C反应蛋白、血沉、疾病活动度评分及IL-17 mRNA呈显著负相关性(P<0.05)。在CD患者中,BATF2 mRNA与C反应蛋白、疾病活动度评分及IL-17 mRNA呈显著负相关性(P<0.05)。美沙拉嗪联合康复新液治疗后UC和CD患者BATF2 mRNA表达量显著高于治疗前,差异有统计学意义(P<0.05)。结论BATF2在IBD患者中异常下降,并与疾病活动性、炎症水平及L-17呈显著性相关性,提示其可能成为IBD的生物标志物。美沙拉嗪联合康复新液治疗可显著增加肠粘膜中BATF2的表达。Objectives To investigate the effect of Mesalazine combined with Kangfuxin Solution on the expression of basic leucine zipper transcription factor ATF-like 2(BATF2)in intestinal mucosa of Patients with Inflammatory Bowel Disease(IBD).Methods A total of 76 IBD patients admitted to our hospital from May 2019 to August 2022 were selected as the observation group,including 38 patients with ulcerative colitis(UC)and 38 patients with Crohn's disease(CD),while 30 healthy individuals who had no colonic lesions during colonoscopy were selected as the normal control group.Fresh intestinal mucosal tissues of patients in the observation group and control group were collected,and intestinal mucosa tissues after treatment with Mesalazine combined with Kangfuxin solution were collected.Fluorescence quantitative PCR was used to measure the expression levels of BATF mRNA,interleukin-17(IL-17)mRNA,and interleukin-10(IL-10)mRNA.Results BATF mRNA in intestinal mucosa of UC and CD patients was significantly lower than that of the control group,IL-17 mRNA was significantly higher than that of the control group,while IL-10 mRNA was significantly lower than that of the control group(P<0.05).In UC patients,BATF mRNA was significantly negatively correlated with C-reactive protein,erythrocyte sedimentation rate,disease activity score,and IL-17 mRNA(P<0.05).In CD patients,BATF mRNA was significantly negatively correlated with C-reactive protein,erythrocyte sedimentation rate,disease activity score,and IL-17 mRNA(P<0.05).After treatment of Mesalazine combined with Kangfuxin solution,the expression of BATF mRNA in UC and CD patients was significantly higher than that before treatment(P<0.05).Conclusions BATF2 is abnormally decreased in IBD patients,and is significantly correlated with disease activity,inflammation level and L-17,suggesting that it can be a biomarker for IBD.Mesalazine combined with Kangfuxin solution can significantly increase the expression of BATF2 in intestinal mucosa.

关 键 词：炎症性肠病 BATF2 美沙拉嗪 康复新液 

分 类 号：R574[医药卫生—消化系统]