miR-29a通过调控Plexin-A1抑制胶质瘤U87细胞增殖并促进凋亡的机制分析  

Mechanism Analysis of miR-29a Inhibits Glioma U87 Cell Proliferation and Promotes Apoptosis by Regulating Plexin-A1

作　　者：杨寒 宁波 YANG Han;NING Bo(Guangzhou Red Cross Hospital of Ji’nan University,Guangzhou City,Guangdong Province 510220)

机构地区：[1]暨南大学附属广州红十字会医院神经外科,广东省广州市510220

出　　处：《医学理论与实践》2024年第4期549-552,共4页The Journal of Medical Theory and Practice

摘　　要：目的:探讨miR-29a调控胶质瘤U87细胞增殖和凋亡的分子机制。方法:qPCR法检测人脑正常胶质HEB细胞与胶质瘤U87细胞中miR-29a和神经丛蛋白A1(Plexin-A1)的mRNA水平,免疫印迹法检测两组细胞中神经丛蛋白A1的蛋白质水平;将胶质瘤U87细胞分为3组,对照组不处理,miR-NC组转染阴性mimic,miR-29a组转染miR-29a-3p mimic;qPCR法检测3组胶质瘤U87细胞中miR-29a的表达情况,CCK8法检测miR-NC组和miR-29a组细胞增殖能力,qPCR法检测miR-NC组和miR-29a组细胞中Plexin-A1的mRNA表达水平,免疫印迹法检测miR-NC组和miR-29a组细胞中Plexin-A1的蛋白表达。结果:qPCR法和WB法结果显示:与HEB细胞相比,胶质瘤U87细胞中miR-29a低表达(P<0.001),Plexin-A1的mRNA(P<0.05)和蛋白质(P<0.05)均高表达;qPCR结果显示:转染后miR-29a组细胞较miR-NC组的miR-29a表达量显著升高(P<0.001),miR-NC组较对照组miR-29a水平未改变(P>0.05);CCK8、流式细胞学结果显示:与miR-NC组相比,miR-29a组细胞相对增殖能力降低(P<0.05)、细胞凋亡率增加6.52%(P<0.05);qPCR和WB结果显示:与miR-NC组相比,miR-29a组细胞中Plexin-A1的mRNA(P<0.05)和蛋白质均降低(P<0.01)。结论:miR-29a-3p可能通过调控神经丛蛋白A1的表达抑制胶质瘤增殖,促进凋亡。Objective:To investigate the molecular mechanism of miR-29a regulating the proliferation and apoptosis of glioma U87 cell.Methods:The mRNA levels of miR-29a and Plexin-A1 in human brain normal glial HEB cells and glioma U87 cells were detected by qPCR,and the protein level of Plexin-A1 in two groups of cells was detected by Western blotting.Glioma U87 cells were divided into three groups.The control group was not treated,the miR-NC group was transfected with negative mimic,and the miR-29a group was transfected with miR-29a-3p mimic.The expression of miR-29a in glioma U87 cells of the three groups was detected by qPCR,the proliferation of miR-NC group and miR-29a group was detected by CCK8 method,and the mRNA expression level of Plexin-A1 in miR-NC group and miR-29a group was detected by qPCR.Western blot was used to detect the protein expression of Plexin-A1 in miR-NC group and miR-29a group.Results:The results of qPCR and WB showed that the expression of miR-29a in glioma U87 cells was lower than that in HEB cells(P<0.001),mRNA of Plexin-A1(P<0.05)and protein(P<0.05).qPCR results showed that the expression of miR-29a in miR-29a group was significantly higher than that in miR-NC group(P<0.001),the level of miR-29a in miR-NC group did not change compared with control group(P>0.05);CCK8 and flow cytometry results showed that compared with miR-NC group,the relative proliferation ability of miR-29a group was decreased(P<0.05),the apoptosis rate increased by 6.52%(P<0.05).The results of qPCR and WB showed that Plexin-A1 mRNA in miR-29a group was significantly higher than that in miR-NC group(t=4.098,P<0.05)and protein(P<0.01).Conclusion:miR-29a-3p may inhibit glioma proliferation and promote apoptosis by regulating the expression of Plexin-A1.

关 键 词：胶质瘤 微小RNA 神经丛蛋白 

分 类 号：R739.41[医药卫生—肿瘤]