蓝莓黑醋栗枸杞决明子复合物对眼部细胞氧化应激损伤的保护作用  

Protective Effect of a Complex of Blueberry,Blackcurrant,Medlar and Cassia Obtusifolia Against Oxidative Stress Injury in Retinal Epithelial Cells

作　　者：王欢 王敏 刘竹青 何运辉 石举然 邹圣灿 WANG Huan;WANG Min;LIU Zhuqing;HE Yunhui;SHI Juran;ZOU Shengcan(Qingdao Chenlan Health Industry Group Co.Ltd.,Qingdao 266000,China)

机构地区：[1]青岛琛蓝健康产业集团有限公司,山东青岛266000

出　　处：《现代食品科技》2024年第1期47-53,共7页Modern Food Science and Technology

摘　　要：探讨了蓝莓黑醋栗枸杞决明子复合物(Blueberry,Blackcurrant,Medlar,Cassia Seed Complex,LHGJ)对丙酮醛(Methylglyoxal,MGO)诱导的人视网膜上皮细胞(RetinalPigmentEpithelium,APRE-19)氧化应激损伤的保护作用。以MGO诱导ARPE-19细胞氧化应激损伤建立细胞模型,给予不同质量浓度LHGJ复合物处理。利用CCK-8法检测细胞活力,采用荧光显微镜检测细胞活性氧(ReactiveOxygenSpecies,ROS)含量、采用酶联免疫法(Enzyme-linked ImmunosorbentAssay,ELISE)测定一氧化氮(Nitric Oxide,NO)含量、比色法测定谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)及羟胺法测定超氧化物歧化酶(Superoxide Dismutase,SOD)活性。实验结果表明,ARPE-19细胞可经0.80 mmol/L MGO诱导氧化应激损伤模型。诱导ARPE-19细胞经0.10、1.00μg/mL LHGJ复合物处理后,细胞活力增加23.03%与21.23%,具有高度显著差异(P<0.001)。0.01、0.10、10.00μg/mL LHGJ复合物使ROS的荧光强度显著降低1.02、1.28、2.37倍(P<0.05),0.01、0.10、10μg/mL LHGJ复合物使NO的生成量显著降低13.24、15.23、16.24μmol/g prot(P<0.05);1.00、10.00μg/mL LHGJ复合物使SOD活性显著与极显著升高6.01、7.43 U/mg(P<0.05、P<0.01);1.00与10.00μg/mL LHGJ复合物显著增加了GSH-Px活力14.32及25.42 U/mg(P<0.05)。LHGJ复合物对MGO导致的ARPE-19细胞氧化应激损伤具有保护作用,可为LHGJ进一步开发提供理论依据。To study the protective effect of blueberry,blackcurrant,medlar,cassia seed complex(LHGJ)against the oxidative stress injury in human retinal epithelial cells(APRE-19)induced by methylglyoxal(MGO).The ARPE-19 cell model was established through inducing oxidative stress by MGO,and LHGJ solutions at different concentrations were used to treat the cells.Cell viability was determined by the CCK-8 method,the reactive oxygen species(ROS)content in cells was measured by the fluorescence microscopy method,the nitric oxide(NO)content was measured by enzyme-linked immunosorbent assay(ELISA),the glutathione peroxidase(GSH-PX)content was measured by colorimetry,and the superoxide dismutase(SOD)activity was measured by the hydroxylamine method.The results showed that oxidative stress injury in ARPE-19 cells could be induced by 0.80 mmol/L MGO.The induced ARPE-19 cells were then treated with 0.10 or 1.00μg/mL LHGJ complex,which increased the cell viability by 23.03%and 21.23%,respectively(P<0.001).LHGJ complex solutions at 0.01,0.10 and 10.00μg/mL significantly(P<0.05)reduced the fluorescence intensity of ROS(by 1.02,1.28 and 2.37 times,respectively),LHGJ complex solutions at 0.01,0.10 and 10.00μg/mL significantly(P<0.05)decreased NO production(by 13.24,15.23 and 16.24μmol/g prot.,respectively),LHGJ complex solutions at 1.0 and 10.00μg/mL significantly(P<0.05)increased the activity of SOD(by 6.01 and 7.43 U/mg,respectively)(P<0.05,P<0.01)and the content of GSH-Px(by 14.32 and 25.42 U/mg,respectively)(P<0.05).LHGJ complex can protect ARPE-19 cells against oxidative stress injury induced by MGO,which provides a theoretical basis for further development of LHGJ complex.

关 键 词：眼部 氧化应激 保护作用 

分 类 号：TS201.4[轻工技术与工程—食品科学]