防风多糖人工胃液代谢产物分析  

Analysis of metabolites in simulated gastric fluid of Saposhnikoviae Radix polysaccharides

作　　者：李洁[1] 徐畅 孙萌[1] 苏成园 靖淑瑾 姜艳艳[1,2] 刘斌 LI Jie;XU Chang;SUN Meng;SU Cheng-yuan;JING Shu-jin;JIANG Yan-yan;LIU Bin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Key Laboratory of"Discovery of Effective Substances in Classical Prescriptions of Traditional Chinese Medicine",National Administration of Traditional Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]国家中医药管理局“中药经典名方有效物质发现”重点研究室,北京102488

出　　处：《中国中药杂志》2024年第2期498-508,共11页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82074283)。

摘　　要：为了深入了解防风多糖能否在胃液中代谢及代谢情况,该文以防风多糖的人工胃液代谢产物为研究对象,结合斑马鱼免疫活性评价,采用高效液相色谱-离子阱-飞行时间质谱(HPLC-IT-TOF-MS)技术系统分析了防风多糖的人工胃液代谢产物。根据所获得的精确相对分子质量、色谱保留行为、质谱裂解规律、对照品比对以及相关文献报道,共分析鉴定了19种代谢产物。其中,代谢产生的单糖5种,寡糖14种。胃液代谢产物中可以准确鉴定出多种还原糖,包括甘露糖、葡萄糖、鼠李糖以及木糖。斑马鱼药效评价结果显示,防风多糖经过胃液代谢后仍具有较好的免疫活性,W3(人工胃液代谢2 h)提高免疫细胞密度的作用最为显著。其中,胃液代谢物M1和M3(Hex-Hex-Man)可能与药理活性关系最为密切,可将其作为潜在的活性片段进行进一步研究。To fully understand whether Saposhnikoviae Radix polysaccharides(SP) can be metabolized in gastric fluid and the meta-bolic behavior,this study systematically analyzed the metabolites in simulated gastric fluid of SP by high-performance liquid chromatography-ion trap time-of-flight mass spectrometry(HPLC-IT-TOF-MS) technology in combination with zebrafish immune activity evaluation.Based on the obtained accurate relative molecular mass,chromatographic retention behavior,MS fragmentation patterns,refe-rence standards,and relevant literature reports,19 metabolites were analyzed and identified.Among them,five monosaccharides and 14 oligosaccharides were generated as metabolites.Several reducing sugars,including mannose,glucose,rhamnose,and xylose,were accurately identified in the gastric fluid metabolites.Zebrafish pharmacological evaluation results indicated that SP maintained good immune activity after gastric fluid metabolism,with the most significant increase in immune cell density observed at W3(simulated gastric fluid metabolism for 2 hours).Among the gastric fluid metabolites,M1 and M3(Hex-Hex-Man) may be most closely related to pharmacological activity and could be further studied as potential active fragments.

关 键 词：防风 多糖 人工胃液 代谢产物 质谱鉴定 

分 类 号：R285[医药卫生—中药学]