超高相液相色谱串联质谱法分析鸡蛋中金刚乙胺含量残留  

Determination of Rimantadine Residues in Eggs by Ultra High Phase Liquid Chromatography Tandem Mass Spectrometry

作　　者：林柯璐 王玲 舒畅 潘志明 LIN Kelu;WANG Ling;SHU Chang;PAN Zhiming(Inspection Center of Food and Drug in Deyang,Deyang 618000;Key Laboratory of Food Inspectionin Deyang,Deyang 618000;Deyang City Luojiang District Food Quality and Safety Supervision Checkpoint,Luojiang 618500)

机构地区：[1]德阳市食品药品安全检验检测中心,德阳618000 [2]德阳市食品检验重点实验室,德阳618000 [3]德阳市罗江区粮食质量安全监督检查站,罗江618500

出　　处：《食品工业》2024年第1期260-263,共4页The Food Industry

摘　　要：采用超高效液相色谱串联质谱法(UPLC-MS/MS)对鸡蛋中金刚乙胺的含量残留进行研究。样品由1%乙酸乙腈提取后,通过PCX阳离子交换固相萃取柱净化,使用Eclipse Plus C_(18)反相色谱柱(2.1 mm×50 mm,1.8μm)进行分离,以0.1%甲酸水和甲醇溶液作为流动相进行梯度洗脱,使用超高效液相色谱-三重四极杆串联质谱联用仪,选择正离子多反应监测模式进行定性定量测定。金刚乙胺在1.0~20.0 ng/mL质量浓度下呈良好线性关系,相关系数(R^(2))大于0.999,分别添加质量分数1,2和10μg/kg这3个水平的标准品,加标回收率为84.2%~91.8%,方法定量限为1.0μg/kg。该方法的建立对于监控鸡蛋中的金刚乙胺残留具有积极作用。The contents of rimantadine in eggs were studied by ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).After the sample was extracted from 1%acetonitrile,it was purified by PCX Cation-exchange solid phase extraction column,separated by Eclipse Plus C_(18) reverse phase column(2.1 mm×50 mm,1.8μm),and gradient elution was performed with 0.1%formic acid water and methanol solution as mobile phase.Using ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry,the positive ion multi-reaction monitoring mode was selected for qualitative and quantitative determination.Rimanantine showed a good linear relationship at the concentration of 1.0-20.0 ng/mL,and the correlation coefficient(R^(2))was greater than 0.999.The standard substances with mass concentrations of 1,2 and 10μg/kg were added,respectively.The recoveries were 84.2%-91.8%,and the limits of quantitation were 1.0μg/kg.The establishment of this method had a positive effect on the monitoring of rimantadine residues in eggs.

关 键 词：超高相液相色谱-串联质谱 金刚乙胺 鸡蛋 

分 类 号：O657.63[理学—分析化学] TS253.7[理学—化学]