2021年贵州省食源性疾病主动监测分离沙门菌耐药及分型特征  

Drug resistance and typing characteristics of Salmonella isolated from foodborne diseases in the Guizhou Province in 2021

作　　者：周倩[1] 黄靖宇[1] 向婧姝 张德著[1] 钟宇婧 朱姝[1] 刘英[1] 李世军[1] 周藜[1] ZHOU Qian;HUANG Jingyu;XIANG Jingshu;ZHANG Dezhu;ZHONG Yujing;ZHU Shu;LIU Ying;LI Shijun;ZHOU Li(Guizhou Center for disease Control and Prevention,Guizhou Guiyang 550004,China;School of Public Health and Wellness,Guizhou Medical University,Guiyang Guizhou 550025,China)

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004 [2]贵州医科大学公共卫生与健康学院,贵州贵阳550025

出　　处：《中国食品卫生杂志》2023年第10期1406-1415,共10页Chinese Journal of Food Hygiene

基　　金：贵州省科技支撑计划项目(黔科合支撑[2021]一般435号);贵州省疾病预防控制中心人才团队项目(RCJD2105、RCJD2102)。

摘　　要：目的了解2021年贵州省食源性疾病主动监测分离沙门菌的血清型、耐药和分子分型特征。方法对全省2021年食源性疾病主动监测腹泻病例中分离的164株沙门菌采用玻片凝集法进行血清学分型,采用微量肉汤稀释法测定菌株对14种抗生素的最小抑菌浓度(MIC)值,采用脉冲场凝胶电泳(PFGE)进行分子分型。结果164株沙门菌可分为25种血清型,优势血清型为鼠伤寒沙门菌(76,46.34%)、肠炎沙门菌(25,15.24%)和爪哇安纳沙门菌(15,9.15%)。164株沙门菌耐药率为100%,多重耐药率达86.59%;其中对氨苄西林、四环素和萘啶酸耐药率较高,分别为95.12%(156/164)、78.05%(128/164)和63.41%(104/164)。72株鼠伤寒沙门菌PFGE聚类分析后共分为58种指纹图谱,24株肠炎沙门菌有12种指纹图谱,15株爪哇安纳沙门菌有3种指纹图谱。结论贵州省腹泻患者沙门菌血清型种类较多,多重耐药现象严重,PFGE指纹图谱表现出遗传多样性。应加强对沙门菌的耐药监测,尤其是优势血清型鼠伤寒沙门菌的临床用药。Objective The aim of this study was to evaluate the serotype,drug resistance,and molecular typing characteristics of Salmonella isolated from patients with foodborne diseases in Guizhou Province in 2021.Methods A total of 164 Salmonella strains were isolated from patients with diarrhea under the Guizhou Provincial Active Surveillance of Foodborne Diseases program in 2021.These strains were subjected to slide agglutination serotyping test,minimum inhibitory concentration tests with 14 antibiotics,and pulsed-field gel electrophoresis(PFGE)for molecular typing.Results The 164 Salmonella strains were divided into 25 serotypes.The dominant serotypes were S.typhimurium(46.34%,76/164),S.enteritidis(15.24%,25/164),and S.javana(9.15%,15/164).The drug resistance rate of the 164 Salmonella strains was 100%(164/164).The multidrug resistance rate was 86.59%(142/164).Out of the 15 antibiotics tested,resistance was most frequently observed against ampicillin(95.12%,156/164),tetracycline(78.05%,128/164),and nalidixic acid(63.41%,104/164).Seventy-two S.typhimurium,24 S.enteritidis,and 15 S.javana strains were divided into 58,12,and 3 different PFGE clusters,respectively.Conclusion There were many Salmonella serotypes detected in patients with diarrhea in the Guizhou Province,and multidrug resistance was common.PFGE fingerprints demonstrated genetic diversity of the isolated strains.Monitoring of drug resistance in Salmonella should be strengthened,especially for the clinical treatment of S.typhimurium.

关 键 词：沙门菌 血清型 耐药 脉冲场凝胶电泳 贵州省 

分 类 号：R155[医药卫生—营养与食品卫生学]