肉苁蓉不同提取部位对db/db小黑鼠降血糖作用  

Hypoglycemic effect of different extracts of Cistanche deserticola on db/db mice

作　　者：程世赞 廉婧 聂紫璇 苏国明 常源 史辑[1,2] 贾天柱 CHENG Shizan;LIAN Jing;NIE Zixuan;SU Guoming;CHANG Yuan;SHI Ji;JIA Tianzhu(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning TCM Processing Engineering Technology Research Center,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药炮制技术产业创新中心,辽宁大连116600

出　　处：《药学研究》2024年第1期6-14,共9页Journal of Pharmaceutical Research

基　　金：国家中医药管理局-重点实验室研究领域的中医临床疗效提升项目(No.2100222179);国家自然科学基金(No.81874345);辽宁省自然科学基金-资助面上项目(No.2022-MS-223)。

摘　　要：目的探讨肉苁蓉和酒苁蓉不同提取部位对db/db小黑鼠降血糖的作用及机制。方法富集肉苁蓉和酒苁蓉总多糖、总寡糖和总苷部分,对其含量进行了比较分析。选用db/db小黑鼠为实验动物,给药28 d后,记录小黑鼠体重、进食量、饮水量,检测血糖,采用ELISA检测血清胰岛素(INS)、糖化血红蛋白(HbA1c),肝脏甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD),血浆和尿液中尿酸(UA)、肌酐(Cr)以及尿微量白蛋白(mALB)水平;HE染色方法制备胰腺和肾脏组织病理切片;IHC测定肾脏type-Ⅳ collagen蛋白表达水平。结果肉苁蓉多糖部位经过酒蒸后含量下降,肉苁蓉寡糖部位中的甜菜碱经过酒蒸后含量增加,总苷类成分酒蒸后松果菊苷的含量稍有下降,毛蕊花糖苷含量下降,异类叶升麻苷含量上升。与模型组相比,肉苁蓉和酒苁蓉不同提取部位能降低体重、空腹血糖(FBG)、口服葡萄糖耐量(OGTT)、HbA1c、胰岛素抵抗指数(HOMA-IR)、胰岛β细胞分泌指数(HOMA-β)、MDA、LDL-C、UA、Cr以及mALB,INS和HDL-C有上升趋势(P<0.05,P<0.01),且能改善胰腺和肾脏的病理损伤以及type-Ⅳ collagen的表达。结论肉苁蓉和酒苁蓉总苷组降血糖作用较好,酒苁蓉多糖和寡糖也有一定的降血糖作用。Objective To explore the hypoglycemic effect and mechanism of different extracts of Cistanche deserticola and processed C.deserticola on db/db mice.Methods The total polysaccharides,total oligosaccharides and total glycosides of C.deserticola and processed C.deserticola were enriched,and their contents were compared and analyzed.Select db/db mice as experimental animals.After 28 days of administration,record the body weight,food intake,water consumption,blood glucose,serum insulin(INS),glycosylated hemoglobin(HbA1c),liver triglyceride(TG),total cholesterol(TC),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C),malondialdehyde(MDA),superoxide dismutase(SOD),uric acid(UA)in plasma and urine creatinine(Cr)and urinary microalbumin(mALB)levels;Pathological sections of pancreas and kidney were prepared by HE staining;The expression level of type-Ⅳ collagen protein in kidney was determined by immunohistochemistry.Results The content of polysaccharide in C.deserticola decreased after wine steaming,the content of betaine in the polysaccharide in C.deserticola increased after wine steaming,the content of total glycosides decreased slightly after wine steaming,the content of verbascoside decreased,and the content of isoacteoside increased.Compared with the model group,the fasting blood glucose(FBG),oral glucose tolerance(OGTT),HbA1c,insulin resistance index(HOMA-IR),HOMA-β,MDA,LDL-C,UA,Cr and mALB in the different extracts of C.deserticola and processed C.deserticola were significantly decreased,INS and HDL-C showed an upward trend(P<0.05,P<0.01),and can improve pathological damage to the pancreas and kidneys,as well as the expression of type-Ⅳ collagen.Conclusion The total glycosides of C.deserticola and processed C.deserticola have better hypoglycemic effects,while polysaccharides and oligosaccharides of processed C.deserticola also have certain hypoglycemic effects.

关 键 词：肉苁蓉 总多糖 总寡糖 总苷 db/db小黑鼠 降血糖 

分 类 号：R285.5[医药卫生—中药学]