金莲花多糖提取工艺优化及其抗氧化活性评价  

Optimization extraction process and evaluation of antioxidant activity of polysaccharides from Trollius chinensis Bunge

作　　者：李春晓 宋庆琳 焦旭[1] 冯慧程 陈虞超[2] 郭生虎[2] 张波[1] 薛涛[1] LI Chunxiao;SONG Qinglin;JIAO Xu;FENG Huicheng;CHEN Yuchao;GUO Shenghu;ZHANG Bo;XUE Tao(College of Medicine,Linyi University,Linyi 276000,China;Agricultural Biotechnology Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China)

机构地区：[1]临沂大学医学院,山东临沂276000 [2]宁夏农林科学院农业生物技术研究中心,宁夏银川750002

出　　处：《药学研究》2024年第1期24-29,共6页Journal of Pharmaceutical Research

基　　金：宁夏自然科学基金(No.2020AAC03296);宁夏固原市科技研发项目(No.2020GYKYF035);宁夏农林科学院农业生物技术研究中心自主创新项目(No.NKYSW-20-1)。

摘　　要：目的优化金莲花多糖提取工艺,并分析测试金莲花多糖抗氧化活性。方法采用回流提取法提取金莲花多糖,通过单因素考察料液比、提取温度、提取时间3个因素对金莲花多糖提取率的影响,并在此基础上通过正交试验设计对提取工艺进行优化。以DPPH自由基、ABTS^(+)自由基、羟基自由基、超氧阴离子清除能力和总还原力为指标评价金莲花多糖的抗氧化活性。结果金莲花多糖的最佳提取工艺为料液比1∶25、提取温度80℃、提取时间2 h,在此条件下金莲花多糖的提取率为1.350%±0.125%。体外抗氧化实验结果表明,金莲花多糖其DPPH自由基、ABTS^(+)自由基、羟基自由基和超氧阴离子自由基清除率IC_(50)分别为0.161、0.079、0.006、4.216 mg·mL^(-1),且当浓度为4.0 mg·mL^(-1)时,总还原力为2.78。结论优化的金莲花多糖提取工艺提取率较高且获得的金莲花多糖具有较好的抗氧化活性,对金莲花多糖的进一步开发利用提供了参考依据。Objective To optimize the extraction method of polysaccharides from Trollius chinensis Bunge and evaluate the antioxidant activity of them.Methods The polysaccharides from Trollius chinensis Bunge were extracted by hot water reflux extraction method,and the effects of solid-liquid ratio,extraction temperature and extraction time on polysaccharides were investigated by a single factor test.On this basis,the orthogonal experimental design method was used to optimize the extraction conditions.The antioxidant activity of polysaccharides from Trollius chinensis Bunge were evaluated by measuring DPPH free radical,ABTS^(+)free radical,hydroxyl free radical,superoxide anion free radical scavenging ability and total reducing power.Results The results showed that the optimal extraction process conditions of polysaccharides from Trollius chinensis Bunge were as follow:solid-liquid ratio of 1∶25,extraction temperature of 80℃,and extraction time of 2 h.Under these conditions,the actual polysaccharides from Trollius chinensis Bunge was 1.350%±0.125%.The IC_(50) values of the scavenging rates of DPPH free radical,ABTS^(+)free radical,hydroxyl free radical and superoxide anion free radical were 0.161,0.079,0.006 and 4.216 mg·mL^(-1),respectively.While,when the concentration of polysaccharides was 4.0 mg·mL^(-1),the total reducing power was 2.78.Conclusion The optimized polysaccharides had an excellent antioxidant activity,which provided a theoretical basis for further exploitation and utilization of polysaccharides from Trollius chinensis Bunge.

关 键 词：金莲花 金莲花多糖 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]