绿豆象成虫对红豆与绿豆种子挥发物的电生理和嗅觉行为反应  

Electrophysiological and olfactory behavioral responses of adult Callosobruchus chinensis(Coleoptera:Chrysomelidae)to the volatiles from Vigna angularis and V.radiata seeds

作　　者：樊楠楠 黄金遇 刘松 张凯 张仙红[1] 郑海霞[1] FAN Nan-Nan;HUANG Jin-Yu;LIU Song;ZHANG Kai;ZHANG Xian-Hong;ZHENG Hai-Xia(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学植物保护学院,太谷030801

出　　处：《昆虫学报》2024年第1期58-67,共10页Acta Entomologica Sinica

基　　金：国家重点研发计划子课题(2021YFD1600603-01);国家现代农业产业技术体系(CARS-08-G10);山西省自然科学基金项目(20210302124166);山西省现代农业产业技术体系建设(杂粮)(2022-03);博士科研启动项目(2015ZZ11)。

摘　　要：【目的】明确红豆Vigna angularis与绿豆V.radiata种子挥发物对绿豆象Callosobruchus chinensis成虫嗅觉行为的影响,为筛选绿豆象行为调控剂奠定理论基础。【方法】利用固相微萃取(solid-phase microextraction,SPME)和气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)法对绿豆晋绿1号和红豆汾小豆3号种子的挥发性化合物进行提取鉴定,通过触角电位(electroantennogram,EAG)和嗅觉选择行为反应筛选对绿豆象具有引诱活性的化合物及配方,并进行田间诱捕试验。【结果】红豆和绿豆种子中的共有挥发物有18种,主要包含醛类、酮类、烯烃类、烷烃类、醇类、酯类和芳香烃类,其中含量较高的4种物质甲基庚烯酮、己醛、正壬醛和癸醛均能引起绿豆象成虫强烈的电生理反应,而正癸烷、反式石竹烯、2-甲基萘和环己基苯等引起的反应相对微弱。嗅觉选择行为反应结果表明己醛对绿豆象雌雄成虫有明显驱避作用;而甲基庚烯酮、正壬醛和癸醛对绿豆象雌雄成虫均表现出引诱作用。多组分室内行为反应试验表明,甲基庚烯酮与正壬醛以1∶2.6的比例混配时雌雄成虫选择率达到70%以上,引诱效果相对较好;而甲基庚烯酮与癸醛以1∶3.2的比例混配时雌雄成虫选择率还未达到60%,引诱效果较差。【结论】红豆与绿豆种子挥发物中甲基庚烯酮、正壬醛和癸醛对绿豆象雌雄成虫有较好的引诱活性,可能在绿豆象嗅觉识别过程中发挥重要作用,可以用于开发该害虫的行为调控剂。【Aim】To clarify the effects of the volatiles from Vigna angularis and V.radiata seeds on the olfactory behavior of adult Callosobruchus chinensis,and to lay a theoretical foundation for the screening of behavioral regulators of C.chinensis.【Methods】The volatile compounds from the seeds of V.radiata Jinlv 1 and V.angularis Fenxiaodou 3 were collected and identified by solid-phase microextraction(SPME)and gas chromatography-mass spectrometry(GC-MS).Based on the electroantennogram(EAG)and olfactory selective behavioral responses,the compounds and formulations with attractiveness were selected,and field trapping experiments were conducted.【Results】There were 18 common volatile compounds from V.angularis and V.radiata seeds,mainly including aldehydes,ketones,alkenes,alkanes,alcohols,esters and aromatic hydrocarbons,and the four substances with high content,6-methylhept-5-en-2-one,hexanal,nonanal and decanal,could cause strong EAG responses in C.chinensis adults,while decane,trans-caryophyllene,2-methylnaphthalene and cyclohexylbenzene caused relatively weak EAG responses.Olfactory selective behavioral response test showed that hexanal had obviously repellent effects on both female and male adults of C.chinensis,while 6-methylhept-5-en-2-one,nonanal and decanal showed attractiveness to female and male adults of C.chinensis.The results of multi-component laboratory behavioral reaction tests showed that when the mix ratio of 6-methylhept-5-en-2-one and nonanal was 1∶2.6,the selection rates of female and male adults reached more than 70%,indicating relatively better attraction effect.However,the selection rates of female and male adults were not up to 60% when 6-methylhept-5-en-2-one and decanal were mixed in a ratio of 1∶3.2,indicating poor attraction effect.【Conclusion】The main volatile compounds of V.angularis and V.radiata,6-methylhept-5-en-2-one,nonanal and decanal,have good attraction activity against female and male adults of C.chinensis,they may play an important role in the olfactory recogniti

关 键 词：绿豆象 挥发性化合物 触角电位 行为反应 引诱剂 

分 类 号：Q968[生物学—昆虫学]