宫颈癌及宫颈上皮内瘤变患者高危型HPV感染与组织中CHD7、BPTF表达的关系  

Relationship between High-risk HPV Infection and CHD7,BPTF Expression in Tissues of Patients with Cervical Cancer and Cervical Intra-epithelial Neoplasia

作　　者：张汝倩 赵君 马灵云 张然 闫冰 ZHANG Ruqian;ZHAO Jun;MA Lingyun;ZHANG Ran;YAN Bing(Department of Gynecology,the Fourth People's Hospital of Hengshui City,Hengshui,Hebei 053000,China)

机构地区：[1]衡水市第四人民医院妇科,河北衡水053000

出　　处：《转化医学杂志》2023年第5期267-271,共5页Translational Medicine Journal

基　　金：2020年衡水市市级科技计划(2020014043Z)。

摘　　要：目的探讨宫颈癌及宫颈上皮内瘤变(CIN)患者高危型人乳头状瘤病毒(HPV)感染与组织中染色质解旋酶DNA结合蛋白7(CHD7)、溴区结构域转录因子(BPTF)表达的关系。方法将2018年5月—2020年5月进行宫颈活检或手术时取得的104例宫颈癌组织标本作为宫颈癌组,82例CIN组织标本作为CIN组,54例正常宫颈组织标本作为对照组。比较3组CHD7、BPTF、高危型HPV表达及不同临床病理特征宫颈癌患者CHD7、BPTF表达,探讨CHD7和BPTF表达与宫颈癌患者高危型HPV感染和预后关系。结果宫颈癌组、CIN组和对照组CHD7、BPTF和高危型HPV阳性表达率逐渐降低,两两比较差异均有统计学意义(P<0.01)。国际妇产科联盟(FIGO)分期为Ⅲ或Ⅳ期、分化程度为低分化、浸润深度≥肌层1/2、有淋巴结转移、高危型HPV阳性的宫颈癌患者CHD7和BPTF阳性表达率高于FIGO分期为Ⅰ或Ⅱ期、分化程度为中高分化、浸润深度<肌层1/2、无淋巴结转移、高危型HPV阴性的宫颈癌患者(P<0.05,P<0.01)。Spearman相关性分析结果显示,CHD7、BPTF表达与宫颈癌患者高危型HPV感染均呈正相关(r=0.407、0.469,P<0.001)。随访3年,104例宫颈癌总生存率为43.27%(45/104)。CHD7阴性表达(76.19%,16/21)宫颈癌患者的3年生存率高于CHD7阳性表达(34.94%,29/83)宫颈癌患者(P<0.01);BPTF阴性表达(77.78%,14/18)宫颈癌患者的3年生存率高于BPTF阳性表达(36.05%,31/86)宫颈癌患者(P<0.01)。结论CHD7和BPTF在宫颈癌患者中高表达,且与高危型HPV感染和预后存在关联。Objective To investigate the relationship between high-risk human papillomavirus(HPV)infection and expression of chromatin helicase DNA-binding protein 7(CHD7)and bromine domain transcription factor(BPTF)in patients with cervical cancer and cervical intra-epithelial neoplasia(CIN).Methods A total of 104 cervical cancer tissue specimens obtained during cervical biopsy or operation from May 2018 to May 2020 were selected as cervical cancer group,82 tissue specimens of CIN were selected as CIN group,and and 54 normal cervical tissue specimens were selected as control group.The expression of CHD7,BPTF and high-risk HPV in the three groups and the expression of CHD7 and BPTF in cervical cancer patients with different clinicopathological characteristics were compared to explore the relationship of CHD7 and BPTF expression with high-risk HPV infection and prognosis in patients with cervical cancer.Results The positive expression rates of CHD7,BPTF and high-risk HPV gradually decreased in cervical cancer group,CIN group and control group,and the differences between the two groups were statistically significant(P<0.01).The positive expression rates of CHD7 and BPTF in patients with stageⅢorⅣcervical cancer based on the International Federation of Obstetrics and Gynecology(FIGO),low differentiation,invasion depth≥1/2 of muscle layer,lymph node metastasis and high-risk type HPV-positive cervical cancer were higher than those with stageⅠorⅡby FIGO staging,medium and high differentiation,invasion depth<1/2 of muscle layer,no lymph node metastasis and high-risk type HPV-negative cervical cancer(P<0.05,P<0.01).Spearman correlation analysis showed that the expression of CHD7 and BPTF were positively correlated with high-risk HPV infection in cervical cancer patients(r=0.407,0.469,P<0.001).After 3 years of follow-up,the overall survival rate of 104 cervical cancer patients was 43.27%(45/104).The 3-year survival rate of cervical cancer patients with negative expression of CHD7(76.19%,16/21)was higher than that of cervica

关 键 词：宫颈肿瘤 宫颈上皮内瘤变 人乳头状瘤病毒 染色质解旋酶DNA结合蛋白7 溴区结构域转录因子 Spearman相关性分析 预后 生存率 

分 类 号：R737.33[医药卫生—肿瘤]