伊姆裸甲藻溶藻菌LD-B3的分离鉴定及溶藻作用  

Isolation and characterization of an algicidal bacterium LD-B3 against the bloom-forming algae Gymnodinium impudicum

作　　者：李俊佳 陈磊 王君玥 蔡月凤 申欣 姬南京 LI Junjia;CHEN Lei;WANG Junyue;CAI Yuefeng;SHEN Xin;JI Nanjing(Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Marine Resources Development Research Institute,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学、江苏省海洋生物资源与环境重点实验室、江苏省海洋生物技术重点实验室,江苏连云港222005 [2]江苏海洋大学、江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [3]江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《应用海洋学学报》2024年第1期192-200,共9页Journal of Applied Oceanography

基　　金：国家重点研发计划(2020YFD0900203);江苏省农业科技自主创新项目[CX(22)2032];国家自然科学基金青年基金(42006135);江苏高校优势学科建设工程项目。

摘　　要：近年来,有害藻华频繁发生给海洋生态系统甚至人类健康带来严重影响。伊姆裸甲藻(Gymnodinium impudicum)是一种典型有害藻华原因种,开展其溶藻菌筛选工作,将有利于该种藻华的防控研究。本研究从伊姆裸甲藻藻华发生海域,分离一株针对藻华原因种的溶藻菌LD-B3,16S rDNA序列分析表明,该菌株属于假交替单胞菌属(Pseudoalteromonas)。菌藻共培养结果显示,2.0%终浓度LD-B3菌液添加72 h后,伊姆裸甲藻溶藻率达81.06%,且溶藻效应呈现浓度和时间依赖性。显微观察和生理参数测定结果表明,菌株LD-B3可导致伊姆裸甲藻细胞链断裂,最大光合效率(Fv/Fm)下降。溶藻方式研究发现,菌株LD-B3主要通过分泌胞外物质实现溶藻,且这类物质对温度较为敏感,初步推断其可能为蛋白类物质。本研究结果表明菌株LD-B3在伊姆裸甲藻溶藻剂开发方面有一定应用前景。In recent years,harmful algal blooms(HABs)have caused significant damage to ecosystems and public health around the world,and the bloom occurrences are increasing in frequency and intensity.The dinoflagellate Gymnodinium impudicum is a typical HAB species,isolation and characterization of algicidal bacteria will be beneficial to control its blooms.In this study,isolation of an algicidal bacterium LD-B3 against G.impudicum was performed.16S rDNA analysis showed that LD-B3 belonged to genus Pseudoalteromonas.After 72 h of LD-B3 bacterium culture addition,the algicidal rate reached up to 81.06%after adding with 2.0%at final concentration.As result,algicidal activity was shown both concentration and time dependent.The results of microscopic observation and measurement of physiological parameters showed that strain LD-B3 could lead to the breakage of G.impudicum cell chain and the decrease of F v/F m.Moreover,our results indicated that strain LD-B3’s extracellular compounds were involved in algicidal activity,and these substances,probably a protein compound,were sensitive to temperature.Generally,these findings indicated that the strain LD-B3 is a potential source of bacterial to mitigate G.impudicum blooms.

关 键 词：海洋生物学 有害藻华 伊姆裸甲藻 溶藻菌 假交替单胞菌 

分 类 号：P735[天文地球—海洋生物学]