槲皮素对微重力引起骨髓间充质干细胞衰老的影响研究  

Influence of quercetin on aging of bone marrow mesenchymal stem cellsinduced by microgravity

作　　者：杨玉田 轩莹莹 高玉海 王龙飞 唐汉琴 马智慧 李亮 武艺 陈克明 YANG Yu-tian;XUAN Ying-ying;GAO Yu-hai;WANG Long-fei;TANG Han-qin;MA Zhi-hui;LI Liang;WU Yi;CHEN Ke-ming(School of Pharmacy,Lanzhou University,Lanzhou 730030,China;Basic Medical Laboratory of 940 Hospital of the Joint Logistics Support Force of the People's Liberation Army of China,Lanzhou 730050,China;Gansu Provincial Key Laboratory of Stem Cells and Gene Drugs,Lanzhou 730050,China)

机构地区：[1]兰州大学药学院,甘肃兰州730030 [2]中国人民解放军联勤保障部队第九四〇医院基础医学实验室,甘肃兰州730050 [3]甘肃省干细胞与基因药物重点实验室,甘肃兰州730050

出　　处：《中国药理学通报》2024年第1期38-45,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 81770879);甘肃省自然科学基金项目(No 22JR11RA013);甘肃省青年科技基金计划(22JR5RA020);中国人民解放军联勤保障部队第九四〇医院实验室培育项目(No 2021yxky081);兰州市科技计划项目(No 2023-1-8)。

摘　　要：目的 研究槲皮素对微重力作用下建立的骨髓间充质干细胞衰老模型的影响。方法 用3D回转仪构造骨髓间充质干细胞衰老模型,给予细胞槲皮素后进行相关蛋白检测、氧化指标检测对药物抗衰老作用进行评估研究。结果 与对照组相比微重力组细胞的衰老相关蛋白p21、p16、p53、RB的表达显著性上升、细胞周期蛋白Cyclin D1、核纤层蛋白lamin B1表达显著下降,差异具有统计学意义(P<0.05)。并且微重力组细胞线粒体膜电位明显下降(P<0.05),细胞ROS蓄积量明显上升(P<0.05),SOD含量明显下降(P<0.05),MDA含量明显上升(P<0.05)。相较于微重力组细胞,槲皮素组细胞的衰老相关蛋白p21、p16、p53、RB的表达显著性下降、细胞周期蛋白cyclin D1、核纤层蛋白lamin B1表达显著上升,差异具有统计学意义(P<0.05)。并且槲皮素组细胞线粒体膜电位明显上升(P<0.05),细胞ROS蓄积量明显下降(P<0.05),SOD含量明显上升(P<0.05),MDA含量明显下降(P<0.05)。结论 槲皮素可抗氧化、保护线粒体功能、保护细胞周期正常进行,从而延缓微重力导致的骨髓间充质干细胞衰老。Aim To investigate the effect of quercetin on the aging model of bone marrow mesenchymal stem cells established under microgravity.Methods Using 3D gyroscope,a aging model of bone marrow mesenchymal stem cells was constructed,and after receiving quercetin and microgravity treatment,the anti-aging effect of the quercetin was evaluated by detecting related proteins and oxidation indexes.Results Compared to the control group,the expressions of age-related proteins p21,p16,p53 and RB in the microgravity group significantly increased,while the expressions of cyclin D1 and lamin B1 significantly decreased,with statistical significance(P<0.05).In the microgravity group,mitochondrial membrane potential significantly decreased(P<0.05),ROS accumulation significantly increased(P<0.05),SOD content significantly decreased and MDA content significantly increased(P<0.05).Compared to the microgravity group,the expressions of age-related proteins p21,p16,p53 and RB in the quercetin group significantly decreased,while the expressions of cyclin D1 and lamin B1 significantly increased,with statistical significance(P<0.05).In the quercetin group,mitochondrial membrane potential significantly increased(P<0.05),ROS accumulation significantly decreased(P<0.05),SOD content significantly increased and MDA content significantly decreased(P<0.05).Conclusions Quercetin can resist oxidation,protect mitochondrial function and normal cell cycle,thus delaying the aging of bone marrow mesenchymal stem cells induced by microgravity.

关 键 词：微重力 衰老 骨髓间充质干细胞 槲皮素 氧化应激 lamin B1 线粒体 

分 类 号：R-332[医药卫生] R285.5R329.24R339.38R349.1R852.22