基于Nrf-2/HO-1探究甘草锌对黄褐斑小鼠的治疗作用  

Exploring the therapeutic effect of licorice zinc on chloasma mice via the Nrf-2/HO-1 pathway

作　　者：杜晓霜 刘萍 邓映 杨洪秋 杜宇 DU Xiaoshuang;LIU Ping;DENG Ying;YANG Hongqiu;DU Yu(Medical Cosmetic Center,Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学附属中医医院医学美容中心,四川泸州646000

出　　处：《中国比较医学杂志》2024年第1期80-87,共8页Chinese Journal of Comparative Medicine

基　　金：泸州市科技局(2019-SYF-42)。

摘　　要：目的探讨甘草锌对黄褐斑的治疗作用。方法将36只BALB/c小鼠平均分为空白组、模型组、甘草锌低剂量组、甘草锌中剂量组、甘草锌高剂量组和氨甲环酸组,用100 mJ/cm^(2) UVB照射联合15 mg/kg黄体酮注射进行黄褐斑造模,后对小鼠进行氨甲环酸(0.065 g/kg)与甘草锌低(0.65 g/kg)/中(1.3 g/kg)/高(2.6 g/kg)剂量连续治疗14 d。取皮肤进行HE、Masson-Fontana染色;并检测皮肤和外周血中SOD、MDA、GSP-Px、TNF-α、IL-1β及IL-6含量;皮肤组织中浆蛋白Nrf-2、核蛋白Nrf-2、HO-1表达水平。结果与模型组相比较,高剂量甘草锌组黑色素细胞形成、胶原细胞坏死与炎性浸润减少(P<0.01),MDA、IL-6、IL-1β与TNF-α表达和浆蛋白Nrf-2表达降低(P<0.01),GSP-Px、SOD表达和核蛋白Nrf-2、HO-1表达增加(P<0.01)。结论甘草锌激活Nrf-2/HO-1通路启动HO-1、SOD和GSP-Px高表达抗氧化应激,从而减少黑色素形成。Objective To investigate the therapeutic effect of licorice zinc on melasma.Methods Thirty-six BALB/c mice were equally divided into blank group,model group,licorzinc low-dose group,licorzinc medium-dose group,licorzinc high-dose group and tranexamic acid group.Melasma was induced by 100 mJ/cm^(2) UVB irradiation combined with 15 mg/kg progesterone injection.Mice were treated with tranexamic acid(0.065 g/kg)and low(0.65 g/kg),medium(1.3 g/kg),or high(2.6 g/kg)doses of zinc licorice for 14 days.Skin was taken for HE and Masson-Fontana staining and measurement of SOD,MDA,GSP-Px,TNF-α,IL-1β,IL-6,plasma protein Nrf-2,nuclear protein Nrf-2 and HO-1 expression levels.Results Compared with model group,high-dose licorice zinc group showed decreased melanocyte formation,collagen cell necrosis,and inflammatory infiltration(P<0.01);decreased MDA,IL-6,IL-1β,TNF-αand plasma protein Nrf-2 expression(P<0.01);and increased GSP-Px,SOD and nuclear protein Nrf-2 and HO-1 expression(P<0.01).Conclusions Zinc licorice activates the Nrf-2/HO-1 pathway to initiate high expression of HO-1,SOD and GSP-Px and fight oxidative stress,thereby reducing melanogenesis.

关 键 词：甘草锌 Nrf-2/HO-1通路 黄褐斑 氧化应激 炎症 

分 类 号：R-33[医药卫生]