血清NY-ESO-1自身抗体和MMP1联合检测对食管鳞状细胞癌的诊断价值  

Combined detection of serum NY-ESO-1 autoantibody and MMP1 for the diagnosis of esophageal squamous cell carcinoma

作　　者：伍方财[1] 林逸伟 陈柳伊 许镒洧[2] WU Fangcai;LIN Yiwei;CHEN Liuyi;XU Yiwei(Department of Radiation Oncology,Cancer Hospital of Shantou University Medical College,Guangdong Shantou 515041,China;Department of Clinical Laboratory,Cancer Hospital of Shantou University Medical College,Guangdong Shantou 515041,China)

机构地区：[1]汕头大学医学院附属肿瘤医院放疗科,广东汕头515041 [2]汕头大学医学院附属肿瘤医院检验科,广东汕头515041

出　　处：《现代肿瘤医学》2024年第4期668-671,共4页Journal of Modern Oncology

基　　金：广东省汕头市科技计划医疗卫生项目(编号:200605115266724)。

摘　　要：目的:探讨血清基质金属蛋白酶-1(matrix metalloproteinase-1,MMP1)和纽约食管鳞状细胞癌抗原-1(New York esophageal squamous cell carcinoma 1,NY-ESO-1)自身抗体联合检测在食管鳞状细胞癌中的诊断意义。方法:应用酶联免疫吸附实验检测120例食管鳞状细胞癌患者和120例正常对照血清中MMP1和NY-ESO-1自身抗体的表达水平,采用受试者工作特征(receiver operating characteristic,ROC)曲线评价诊断效能。结果:血清MMP1和NY-ESO-1自身抗体在食管鳞状细胞癌患者中的表达均明显高于正常对照[(8.070±5.738)ng/mL vs(4.331±3.137)ng/mL,Z=6.214,P<0.001;0.463±0.571 vs 0.156±0.086,Z=5.210,P<0.001]。ROC曲线显示,当血清MMP1为最佳诊断临界值10.586 ng/mL时,其在诊断食管鳞状细胞癌的曲线下面积(area under the ROC curve,AUC)为0.732(95%CI:0.671~0.787),敏感度为24.2%,特异度为95.0%。NY-ESO-1自身抗体诊断食管鳞状细胞癌AUC为0.695(95%CI:0.632~0.752),敏感度为33.0%,特异度为95.0%。MMP1和NY-ESO-1自身抗体联合检测诊断食管鳞状细胞癌的AUC为0.800(95%CI:0.744~0.849),敏感度为47.5%,特异度为95.0%。结论:血清MMP1和NY-ESO-1自身抗体联合检测可能有助于提高食管鳞状细胞癌的诊断效能。Objective:To evaluate the diagnostic value of the combination of serum matrix metalloproteinase-1(MMP1)and New York esophageal squamous cell carcinoma 1(NY-ESO-1)autoantibody in patients with esophageal squamous cell carcinoma(ESCC).Methods:The study included 120 patients with ESCC and 120 normal controls.Serum levels of MMP1 and NY-ESO-1 autoantibody were measured by enzyme-linked immunosorbent assay(ELISA).Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficacy.Results:The serum levels of MMP1 and NY-ESO-1 autoantibody were significantly higher in ESCC patients than in normal controls[(8.070±5.738)ng/mL vs(4.331±3.137)ng/mL,Z=6.214,P<0.001;0.463±0.571 vs 0.156±0.086,Z=5.210,P<0.001].ROC curves showed when the optimum diagnostic cutoff of MMP1 was 10.586 ng/mL,the area under the ROC curve(AUC)was 0.732(95%CI:0.671~0.787),with sensitivity of 24.2%,and specificity of 95.0%.Measurement of NY-ESO-1 autoantibody demonstrated an AUC of 0.695(95%CI:0.632~0.752),with a sensitivity and specificity of 33.0%and 95.0%.The combination of MMP1 and NY-ESO-1 autoantibody showed an AUC of 0.800(95%CI:0.744~0.849),a sensitivity of 47.5%and a specificity of 95.0%.Conclusion:Serum MMP1 and NY-ESO-1 autoantibody as potential biomarkers might aid the early diagnosis of ESCC.

关 键 词：基质金属蛋白酶-1 NY-ESO-1自身抗体 食管鳞状细胞癌 诊断标志物 

分 类 号：R735.1[医药卫生—肿瘤]