泉州“过饥草”类植物的种类调查与ITS2序列分析  

Species Survey and ITS2 Sequence Analysis of KèKi Chháu Medicinal Plants in Quanzhou Region

作　　者：林清凡 黄秀珍 郭婷婷 杨亚龙 温扬敏 LIN Qing-fan;HUANG Xiu-zhen;GUO Ting-ting;YANG Ya-long;WEN Yang-min(Medical Research Center,Quanzhou Medical College,Quanzhou 362100,China)

机构地区：[1]泉州医学高等专科学校医学科研中心,福建泉州362100

出　　处：《海峡药学》2024年第1期26-30,共5页Strait Pharmaceutical Journal

基　　金：福建省教育厅中青年教师教育科研项目(编号:JAT171163);泉州市科技计划项目(编号:2020N058S、2018N107S);泉州医学高等专科学校科研项目(编号:XJK1614B)。

摘　　要：目的 采集与鉴定泉州民间一类保胃药用植物“过饥草”(闽南语KèKi Chháu),并选用内转录间隔区2(Internal Transcribed Spacer 2,ITS2)序列作为条形码进行分析。方法 采集泉州区域内“过饥草”类植物样本,经传统形态学鉴定后,提取基因组DNA,测序各样本的ITS和ITS2区域。根据ITS2序列计算各样本间的遗传距离,构建邻接法系统进化树,并预测ITS2序列的二级结构,比较各个ITS2序列二级结构的差异。结果 获取了全部样本的ITS2序列,并将14株采集样本分属于8种植物。而基于ITS2序列的系统进化分析、二级结构比对分析与其形态学鉴定结果一致,可明显区分“过饥草”类植物的物种差异。结论 共梳理出8种“过饥草”类药用植物的物种信息,且ITS2序列可作为鉴别该类民间药用植物的有效条形码。OBJECTIVE To collected a class of gastric protection medicinal plants called Kè-Ki-Chháu in Quanzhou region,and used Internal Transcribed Spacer 2(ITS2)sequence as barcode for molecular identification.METHODS After morphological identification,genomic DNA of collected Kè-Ki-Chháu plants were extracted and performed to obtain the ITS and ITS2 region by PCR amplification.Based on the ITS2 sequences alignment,genetic distances among species were calculated for analyzing phylogenetic tree construction.Then,conformation comparative analysis was applied to reveal the species-divergence of secondary structure of ITS2 sequences.RESULTS 14 collected Kè-Ki-Chháu plants were classified to 8 species(4 families,8 genera).Phylogenetic analysis based on sequences information and secondary structure of ITS2 region,were concurred with the result of morphological classification.CONCLUSION This research classified a total of 8 species information and proved that the ITS2 barcode is capable for phylogenetic identification of homonymous Kè-Ki-Chháu medicinal plants.

关 键 词：“过饥草”类药用植物 系统进化分析 ITS2序列 

分 类 号：R284[医药卫生—中药学]