KIF26B在膀胱癌组织中的表达及对膀胱癌细胞增殖、侵袭、迁移的影响  

Expression of KIF26B in Bladder Cancer Tissues and its Effect on the Proliferation,Invasion and Migration of Bladder Cancer Cells

作　　者：卢保德 龙贤 黄勇平[1] LU Baode;LONG Xian;HUANG Yongping(Department of Urology,Affiliated Hospital of Youjiang Medical College for Nationalities,Guangxi 533000,China)

机构地区：[1]右江民族医学院附属医院泌尿外科,百色533000

出　　处：《医学研究杂志》2024年第1期107-112,共6页Journal of Medical Research

基　　金：广西壮族自治区百色市科学研究与技术开发计划项目(百科20213719);右江民族医学院高层次人才科研项目(R20196337)。

摘　　要：目的基于公共数据库分析驱动蛋白家族成员26B(kinesin family member 26B,KIF26B)在膀胱癌中的表达水平,并探讨沉默KIF26B对膀胱癌细胞增殖、侵袭、迁移的影响。方法基于GEO和UaLcan数据库分析KIF26B在膀胱癌中的表达及与患者生存预后的关系;实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)和Western blot法检测膀胱癌细胞系(T24、J82)及膀胱正常上皮细胞SV-HUV-1中KIF26B的表达水平;将si-KIF26B、si-NC片段转染至T24细胞,采用噻唑蓝(methyl thiazolyl tetrazolium,MTT)法、Transwell实验和划痕实验检测沉默KIF26B后对T24细胞增殖、侵袭和迁移的影响;Western blot法检测沉默KIF26B后丝裂原活化蛋白激酶激酶(mitogen-activated protein kinase kinase,MEK)、磷酸化丝裂原活化蛋白激酶激酶(phosphorylated mitogen-activated protein kinase kinase p-MEK)、细胞外调节蛋白激酶(extracellular signal-regulated kinases,ERK)、磷酸化细胞外调节蛋白激酶(phosphorylated extracellular regulatory protein kinase,p-ERK)蛋白的表达水平。结果KIF26B在膀胱癌组织中高表达,KIF26B高表达患者的预后更差(P<0.05)。KIF26B在膀胱癌细胞系T24、J82中的表达水平显著高于膀胱正常上皮细胞SV-HUV-1(P<0.05)。沉默KIF26B基因后,MTT、Transwell和划痕实验结果显示,T24细胞的增殖、侵袭、迁移能力明显下降(P<0.05);沉默KIF26B后,T24细胞中p-MEK、p-ERK蛋白的表达下调(P<0.05),而MEK、ERK蛋白无明显变化(P>0.05)。结论KIF26B在膀胱癌组织和细胞中高表达,与患者预后不良相关,沉默KIF26B可抑制膀胱癌细胞增殖、侵袭和迁移,其机制可能通过MEK/ERK通路发挥作用。Objective To analyze the expression level of kinesin family member 26B(KIF26B)in bladder cancer based on the public database,and to investigate the effect of silencing KIF26B on the proliferation,invasion and migration of bladder cancer cells.Methods The expression of KIF26B in bladder cancer and its relationship with survival and prognosis were analyzed based on GEO and UaLcan databases.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect the expression of KIF26B in bladder cancer cell lines(T24,J82)and normal bladder epithelial cells SV-HUV-1.si-KIF26B and si-NC fragments were transfected into T24 cells,and the effects of silencing KIF26B on the proliferation,invasion and migration of T24 cells were detected by methyl thiazolyl tetrazolium(MTT)assay,Transwell assay and scratch assay.Western blot was used to detect the expression levels of p-MEK,MEK,ERK and p-ERK proteins after the silencing of KIF26B.Results KIF26B was highly expressed in bladder cancer tissues,and the prognosis of patients with high expression of KIF26B was worse(P<0.05).The expression level of KIF26B in bladder cancer cell lines T24 and J82 was significantly higher than that in normal bladder epithelial cells SV-HUV-1(P<0.05).After the silencing of KIF26B gene,MTT,Transwell and scratch assay Results showed that the proliferation,invasion and migration ability of T24 cells were significantly decreased(P<0.05);After silencing KIF26B,the expression of p-MEK and p-ERK proteins in T24 cells was down-regulated(P<0.05),while MEK and ERK proteins had no significant changes(P>0.05).Conclusion KIF26B is highly expressed in bladder cancer tissues and cells,which is associated with poor prognosis of patients.Silencing KIF26B can inhibit the proliferation,invasion and migration of bladder cancer cells,and the mechanism may play a role through the MEK/ERK pathway.

关 键 词：KIF26B 膀胱癌 增殖 侵袭 迁移 

分 类 号：R737[医药卫生—肿瘤]