副溶血弧菌VP-5的分离鉴定及其与鲈鱼免疫信号通路相互关系研究  

Isolation and Identification of Vibrio parahaemolyticus VP-5 and Its Relationship with Immune Signaling Pathway in Lateolabrax japonicus

作　　者：余文杰[1] 谢建华[1] 林勇文 郑守斌 蓝炎阳 YU Wenjie;XIE Jianhua;LIN Yongwen;ZHENG Shoubin;LAN Yanyang(School of Food&Biological Engineering,Zhangzhou Institute of Technology,Zhangzhou Fujian 363000;Fujian Longmenglong Biotechnology Co.,Ltd.,Zhangzhou Fujian 363000)

机构地区：[1]漳州职业技术学院食品工程学院,福建漳州363000 [2]福建省龙梦龙生物科技有限公司,福建漳州363000

出　　处：《现代农业科技》2024年第4期164-169,共6页Modern Agricultural Science and Technology

基　　金：国家自然科学基金(30900915);福建省科技厅科学研究项目(JK2012012);福建省科技计划项目(2018N2002);漳州市食品产业技术研究院开放课题(ZSY2022109)。

摘　　要：本文以染病鲈鱼为试验材料,对其肝脏的致病菌进行分离、纯化、鉴定,并分析了致病菌与鲈鱼免疫信号通路的相互关系。结果表明,经分离、纯化,从染病鲈鱼肝脏中得到一株菌株VP-5。VP-5呈球杆状、弧形,有单鞭毛,运动活跃,为革兰氏阴性菌,对酪氨酸、葡萄糖、甘露醇等反应阳性,对蔗糖、阿拉伯糖和棉子糖等反应阴性,其16S rRNA和TDH1基因序列与副溶血弧菌的亲缘关系非常接近。经综合鉴定,致病菌VP-5为副溶血弧菌。鲈鱼受到副溶血弧菌VP-5感染后,免疫信号通路基因CD63、CXCR4、IL-8在鲈鱼肝脏组织中12 h和24 h时的相对表达量与无菌生理盐水对照组(CK)相比差异显著(P<0.05);在表皮组织中24 h和36 h时的相对表达量与无菌生理盐水对照组(CK)相比差异显著(P<0.05)。证实鲈鱼肝脏组织免疫信号通路基因的表达与副溶血弧菌VP-5的感染正相关,相对于表皮组织反应更快。鲈鱼的免疫信号通路基因CD63、CXCR4、IL-8积极反应于致病菌,这些基因的快速表达会引发鲈鱼自身免疫应答,增强鲈鱼抵抗致病菌的能力。This paper used infected Lateolabrax japonicus as the experimental material to isolate,purify and identify the pathogenic bacteria in its liver,and analyzed the relationship between the pathogenic bacteria and the immune signaling pathway of Lateolabrax japonicus.The results showed that a strain VP-5 was isolated and purified from infected Lateolabrax japonicus liver.VP-5 was rod-shaped and arc-shaped,with a single flagella and active movement.It was a Gram negative bacterium that reacted positively to tyrosine,glucose,mannitol and other substances,but negatively to sucrose,arabinose and raffinose.Its 16S rRNA and TDH1 gene sequences were closely related to Vibrio parahaemolyticus.After comprehensive identification,the pathogenic bacterium VP-5 was Vibrio parahaemolyticus.After being infected with Vibrio parahaemolyticus VP-5,the relative expression levels of immune signaling pathway genes CD63,CXCR4 and IL-8 in the liver tissue of Lateolabrax japonicus at 12 and 24 hours were significantly different from those in the sterile saline control group(CK)(P<0.05);the relative expression levels in epidermal tissue at 24 and 36 hours showed significant differences compared with the sterile saline control group(CK)(P<0.05).It was confirmed that the expression of immune signaling pathway genes in the liver tissue of Lateolabrax japonicus was positively correlated with the infection of Vibrio parahaemolyticus VP-5,with a faster response compared with the epidermal tissue.The immune signaling pathway genes CD63,CXCR4 and IL-8 in Lateolabrax japonicus actively responded to pathogenic bacteria.The rapid expression of these genes could trigger the self immune response of Lateolabrax japonicus and enhance its ability to resist pathogenic bacteria.

关 键 词：副溶血弧菌 鲈鱼 CD63基因 CXCR4基因 IL-8基因 

分 类 号：S828[农业科学—畜牧学]