Comparative analysis of the cardiac structure and transcriptome of scallop and snail,perspectives on heart chamber evolution  

作　　者：Meina Lu Rabia Hayat Xuejiao Zhang Yaqi Jiao Jianyun Huang Yifan Huangfu Mingcan Jiang Jieyi Fu Qingqiu Jiang Yaojia Gu Shi Wang Alexander A.Akerberg Ying Su Long Zhao 

机构地区：[1]Key Laboratory of Evolution and Marine Biodiversity(Ministry of Education)and Institute of Evolution and Marine Biodiversity,Ocean University of China,Qingdao 266003,China [2]College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [3]College of Fisheries,Ocean University of China,Qingdao 266003,China [4]Fang Zongxi Centre for Marine EvoDevo and MOE Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [5]Division of Basic and Translational Cardiovascular Research,Department of Cardiology,Boston Children’s Hospital,Boston,MA 02115,USA [6]Harvard Medical School,Boston,MA 02115,USA

出　　处：《Marine Life Science & Technology》2023年第4期478-491,共14页海洋生命科学与技术（英文）

基　　金：supported by Laoshan Laboratory(LSKJ202203204);the National Natural Science Foundation of China(31970506 and 32170541 to LZ;31970475 and 32170832 to YS);the Fundamental Research Funds for Central Universities,China(202012004 to LZ).

摘　　要：The evolution of a two-chambered heart,with an atrium and a ventricle,has improved heart function in both deuterostomes(vertebrates)and some protostomes(invertebrates).Although studies have examined the unique structure and function of these two chambers,molecular comparisons are few and limited to vertebrates.Here,we focus on the two-chambered protostome heart of the mollusks,offering data that may provide a better understanding of heart evolution.Specifically,we asked if the atrium and ventricle differ at the molecular level in the mollusk heart.To do so,we examined two very different species,the giant African land snail(Lissachatina fulica)and the relatively small,aquatic yesso scallop(Mizuhopecten yessoensis),with the assumption that if they exhibited commonality these similarities would likely reflect those across the phylum.We found that,although the hearts of these two species differed histologically,their cardiac gene function enrichments were similar,as revealed by transcriptomic analysis.Furthermore,the atrium and ventricle in each species had distinct gene function clusters,suggesting an evolutionary differentiation of cardiac chambers in mollusks.Finally,to explore the relationship between vertebrate and invertebrate two-chambered hearts,we compared our transcriptomic data with published data from the zebrafish,a well-studied vertebrate model with a two-chambered heart.Our analysis indicated a functional similarity of ventricular genes between the mollusks and the zebrafish,suggesting that the ventricle was differentiated to achieve the same functions in invertebrates and vertebrates.As the first such study on protostomes,our findings offered initial insights into how the two-chambered heart arose,including a possible understanding of its occurrence in both protostomes and deuterostomes.

关 键 词：ATRIUM Heart evolution MOLLUSK RNA sequencing VENTRICLE Zebrafish 

分 类 号：Q178.53[生物学—水生生物学] Q95[生物学—普通生物学]