HPV DNA和HPV E6/E7 mRNA检测在宫颈病变筛查中的价值  

The value of HPV DNA and HPV E6/E7 mRNA detection in screening for cervical lesions

作　　者：宋汉韬 刘小娟[1,2] 刘芳[1,2] 常莉[1,2] 滕洁[1,2] SONG Hantao;LIU Xiaojuan;LIU Fang;CHANG Li;TENG Jie(Department of Clinical Laboratory,West China Second University Hospital,Chengdu,Sichuan 610041,China;Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education,Sichuan University,Chengdu,Sichuan 610041,China)

机构地区：[1]四川大学华西第二医院检验科,四川成都610041 [2]四川大学妇儿疾病与出生缺陷教育部重点实验室,四川成都610041

出　　处：《国际检验医学杂志》2024年第4期467-470,共4页International Journal of Laboratory Medicine

基　　金：四川省科技厅重点研发项目(2022YFS0242)。

摘　　要：目的探讨人乳头瘤病毒(HPV)DNA与HPV E6/E7 mRNA检测在宫颈病变筛查中的价值。方法选取2021年9月至2022年8月于四川大学华西第二医院进行宫颈病变筛查的619例患者作为研究对象,以其病理结果为标准,评估HPV DNA与HPV E6/E7 mRNA检测在宫颈病变筛查中的价值。结果619例患者中,HPV DNA检测的阳性率为64.78%(401/619),HPV E6/E7 mRNA检测的阳性率为60.74%(376/619)。宫颈病变筛查主要以30~<40岁和40~<50岁的性活跃人群为主。HPV DNA和HPV E6/E7 mRNA检测的阳性率均随宫颈病变级别的升高而升高。两种方法联合检测的灵敏度、阳性预测值、阴性预测值均高于HPV DNA检测或HPV E6/E7 mRNA检测的结果。结论两种方法联合检测可面向各个病理阶段的宫颈病变筛查,在临床诊疗中能发挥更大的指导价值。Objective To investigate the value of human papillomavirus(HPV)DNA and HPV E6/E7 mRNA detection in screening for cervical lesions.Methods A total of 619 patients who were screened for cervical lesions in West China Second University Hospital from September 2021 to August 2022 were selected as the study objects.The value of HPV DNA and HPV E6/E7 mRNA detection in cervical lesion screening was evaluated based on their pathological results.Results The positive rate of HPV DNA detection was 64.78%(401/619)and that of HPV E6/E7 mRNA detection was 60.74%(376/619)in 619 patients.Cervical lesion screening was mainly conducted in sexually active people aged 30-<40 years and 40-<50 years.The positive rates of HPV DNA and HPV E6/E7 mRNA increased with the increase of cervical lesion grade.The sensitivity,positive predictive value and negative predictive value of the two methods were higher than those of HPV DNA or HPV E6/E7 mRNA detection.Conclusion The combined detection of the two methods can be used for screening cervical lesions at various pathological stages,which can provide greater guidance value in clinical diagnosis and treatment.

关 键 词：宫颈病变 人乳头瘤病毒 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]