野生和不同栽培方式桑黄药材质量分析  

Analysis on the quality of wild and different cultivation methods of Sanghuang porus vaninii (Ljub.) L.W. Zhou & Y.C. Dai

作　　者：吴陈晨 严露 蒲婧哲 张亚中 韩岚 Wu Chenchen;Yan Lu;Pu Jingzhe;Zhang Yazhong;Han Lan(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Institute for Food and Drug Control,Hefei 230051,China;State Drug Administration Key Laboratory of Quality Research and Evaluation of Chinese Medicine,Hefei 230051,China;Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology,Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,合肥230012 [2]安徽省食品药品检验研究院,合肥230051 [3]国家药品监督管理局中药质量研究与评价重点实验室,合肥230051 [4]中药饮片制造新技术安徽省重点实验室,合肥230012

出　　处：《国际中医中药杂志》2024年第2期209-214,共6页International Journal of Traditional Chinese Medicine

基　　金：安徽省科技重大专项(202203a07020014)。

摘　　要：目的通过对桑黄药材的超高效液相色谱(UPLC)特征图谱和多组分含量测定结果进行分析, 比较并评价野生和不同栽培方式桑黄药材质量。方法采用UPLC建立桑黄药材的特征图谱及多组分含量测定方法, 并运用聚类分析、正交偏最小二乘法-判别分析(OPLS-DA)方法进行化学模式识别分析。结果 18批桑黄药材特征图谱有10个共有峰, 指认出麦角硫因(峰1)、原儿茶酸(峰2)、原儿茶醛(峰3)、咖啡酸(峰4)、Hispidin(峰5)5个成分, 聚类分析、OPLS-DA可将野生品及不同栽培方式桑黄药材明确区分。结论段木栽培桑黄较袋料栽培桑黄与野生桑黄更接近, 建立的UPLC特征图谱和多成分含量测定方法可为桑黄药材的质量评价提供参考。Objective To compare and evaluate the quality of wild and different cultivation methods of Sanghuang porus vaninii(Ljub.)L.W.Zhou&Y.C.Dai through analysis on UPLC characteristic atlas and multi-component content determination results.Methods UPLC was used to establish the characteristic chromatogram and multi-component content determination method of Sanghuang porus vaninii(Ljub.)L.W.Zhou&Y.C.Dai,and clustering analysis,orthogonal partial least squares-discriminant analysis method were used for chemical pattern recognition analysis.Results The results showed that there were 10 common peaks in 18 batches of Sanghuang porus vaninii(Ljub.)L.W.Zhou&Y.C.Dai.Five components were identified,erythrothioneine(peak 1),protocatechuic acid(peak 2),protocatechualdehyde(peak 3),caffeic acid(peak 4)and Hispidin(peak 5).HCA and OPLS-DA could distinguish Sanghuang porus vaninii(Ljub.)with different cultivation methods.Conclusion Sanghuang porus vaninii(Ljub.)L.W.Zhou&Y.C.Dai in wood is closer to wild Sanghuang porus vaninii(Ljub.)L.W.Zhou&Y.C.Dai than in substitute cultivation.The UPLC characteristic atlas and multi-component content determination method established in this study can provide reference for the quality evaluation of Sanghuang porus vaninii(Ljub.)L.W.Zhou&Y.C.Dai.

关 键 词：质量控制 超高效液相色谱 桑黄 药用植物栽培 

分 类 号：R284.1[医药卫生—中药学]