基于rbcL序列的辽宁省苍术药材DNA条形码研究  

Study on DNA barcoding of Atractylodes chinensis (DC.) Koidz. herbs from Liaoning Province based on rbcL sequences

作　　者：于莹 赵容[1] 高铭泽 薛嘉宁 尹海波[1] Yu Ying;Zhao Rong;Gao Mingze;Xue Jianing;Yin Haibo(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,大连116600

出　　处：《国际中医中药杂志》2024年第2期249-253,共5页International Journal of Traditional Chinese Medicine

基　　金：辽宁省道地DNA指纹图谱建立项目实施方案(2022003);中央本级重大增减支项目(2060302)。

摘　　要：目的采用rbcL序列对辽宁采集北苍术根茎进行鉴定, 为保证辽宁省道地药材栽培可行性提供依据。方法收集辽宁省10个地区栽培的北苍术根茎共30份, 提取总DNA, 采用PCR筛选DNA条形码, 并对样本的rbcL序列进行扩增、测序, 计算扩增成功率和测序成功率;使用MEGA 7.0软件进行序列比对;使用邻接法(NJ)构建系统聚类树。结果北苍术根茎DNA提取成功率均为93.3%, PCR扩增和测序成功率均为100%。辽宁省30个北苍术样本中, 2个样本存在种内变异, 其余北苍术的碱基序列均完全相同。北苍术与菊科近缘种苍术属药材距离较近, 与菊科其他属植物之间遗传距离较远。NJ树可区分北苍术及其近缘种药材。结论辽宁省北苍术栽培品质量基本相似, rbcL序列可作为北苍术DNA条形码鉴定的有效序列片段。Objective To use rbcL sequences to identify the rhizomes of the Liaoning collection of Atractylodes chinensis(DC.)Koidz.;To provide a basis for ensuring the feasibility of cultivation of the native herb in Liaoning Province.Methods A total of 30 rhizomes of Atractylodes chinensis(DC.)Koidz.were collected from 10 regions cultivated in Liaoning Province,and the total DNA was extracted.DNA barcodes were screened by PCR,and the rbcL sequences of the samples were amplified and sequenced,and the amplification and sequencing success rates were calculated.Sequence alignment was performed using MEGA 7.0 software;a systematic clustering tree was constructed using the neighbour-joining method.Results The success rates of DNA extraction from the rhizomes of Atractylodes chinensis(DC.)Koidz.were all 93.3%,and the success rates of PCR amplification and sequencing were all 100%.Among the 30 samples of Atractylodes chinensis(DC.)Koidz.in Liaoning Province,two samples had intraspecific variation,and the rest of the base sequences of Atractylodes chinensis(DC.)Koidz.were identical.Atractylodes chinensis(DC.)Koidz.was closer to the herbs of the genus Cangzhu,a relative species of Asteraceae,and was genetically more distant from the rest of Asteraceae.The NJ tree could distinguish Atractylodes chinensis(DC.)Koidz.and its relatives.Conclusion The quality of Atractylodes chinensis(DC.)Koidz.cultivars in Liaoning Province is basically similar,and the rbcL sequence can be used as a valid sequence fragment for the identification of Atractylodes chinensis(DC.)Koidz.DNA barcode.

关 键 词：中药鉴定 北苍术 DNA条形码 分类学 RBCL序列 药用植物栽培 

分 类 号：R282.5[医药卫生—中药学]