转录组分析植物促生细菌缓解高粱微塑料和重金属复合污染胁迫机制  

Transcriptome Analysis of Plant Growth-promoting Bacteria Alleviating Microplastic and Heavy Metal Combined Pollution Stress in Sorghum

作　　者：刘泳岐 赵锶禹 任学敏[2] 李玉英[1] 张英君[2] 张浩 韩辉 陈兆进 LIU Yong-qi;ZHAO Si-yu;REN Xue-min;LI Yu-ying;ZHANG Ying-jun;ZHANG Hao;HAN Hui;CHEN Zhao-jin(International Joint Laboratory of Watershed Ecological Security,Collaborative Innovation Center of Water Security for Water Source Region of Middle Route Project of South-North Water Diversion in Henan Province,College of Water Resource and Environment Engineering,Nanyang Normal University,Nanyang 473061,China;School of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang 473061,China)

机构地区：[1]南阳师范学院水资源与环境工程学院,河南省南水北调中线水源区流域生态安全国际联合实验室,南水北调中线水源区水安全河南省协同创新中心,南阳473061 [2]南阳师范学院生命科学与农业工程学院,南阳473061

出　　处：《环境科学》2024年第1期480-488,共9页Environmental Science

基　　金：国家自然科学基金项目(U2004145,41601332);河南省高校科技创新人才项目(23HASTIT018);河南省重点研发专项(221111520600);河南省科技攻关项目(232102111024);河南省中央引导地方科技发展资金项目(Z20221343035);河南省高等学校重点科研项目(23A610003)。

摘　　要：微塑料能成为其它环境污染物(比如重金属)潜在的转运载体,因此微塑料与重金属的复合污染越来越受到研究者的重视.为探究植物促生细菌VY-1缓解高粱微塑料和重金属复合污染胁迫机制,通过水培实验分析菌株接种对高粱生物量和重金属积累的影响,通过转录组学分析接菌对高粱基因表达的影响.结果表明,聚乙烯(PE)和镉(Cd)复合污染相比单一Cd污染使高粱地上部和地下部干重分别下降17.04%和10.36%,表现出复合污染对植物生长毒性效应增强的症状.接种植物促生细菌VY-1能缓解Cd-PE复合污染的毒性,高粱地上部和地下部长度分别增加33.83%和73.21%,干重分别增加56.64%和33.44%.转录组测序结果表明,接种VY-1后高粱根部有904个基因上调表达.促生细菌VY-1接种能上调表达生长素、脱落酸、黄酮类和木质素生物合成通路中多个基因,促进了高粱在Cd-PE复合污染胁迫下的耐受能力,提高其抗性.以上研究结果表明植物促生细菌在微塑料和重金属的复合污染中能通过调节植物基因表达缓解所受到的胁迫,为重金属与微塑料复合污染植物-微生物联合修复提供了参考.Microplastics can become potential transport carriers of other environmental pollutants(such as heavy metals),so the combined pollution of microplastics and heavy metals has attracted increasing attention from researchers.To explore the mechanism of plant growth-promoting bacteria VY-1 alleviating the combined pollution stress of heavy metals and microplastics in sorghum,the effects of inoculation on biomass and accumulation of heavy metals in sorghum were analyzed using a hydroponics experiment,and the effects of inoculation on gene expression in sorghum were analyzed via transcriptomics.The results showed that the combined pollution of polyethylene(PE)and cadmium(Cd)decreased the dry weight of above-ground and underground parts by 17.04% and 10.36%,respectively,compared with that under the single Cd pollution,which showed that the combined toxicity effect of the combined pollution on plant growth was enhanced.The inoculation of plant growth-promoting bacteria VY-1 could alleviate the toxicity of Cd-PE combined pollution and increase the length of aboveground and underground parts by 33.83% and 73.21% and the dry weight by 56.64% and 33.44%,respectively.Transcriptome sequencing showed that 904 genes were up-regulated after inoculation with VY-1.Inoculation with growth-promoting bacteria VY-1 could up-regulate the expression of several genes in the auxin,abscisic acid,flavonoid synthesis,and lignin biosynthesis pathways,which promoted the response ability of sorghum under Cd-PE combined pollution stress and improved its resistance.The above results indicated that plant growth-promoting bacteria could alleviate the stress of heavy metal and microplastic combined pollution by regulating plant gene expression,which provided a reference for plant-microbial joint remediation of heavy metal and microplastic combined pollution.

关 键 词：高粱 镉(CD) 聚乙烯(PE) 根际促生细菌 转录组 

分 类 号：X171.5[环境科学与工程—环境科学]