基于韭UPLC-HESI-MS/MS法的菜坪2号烟叶代谢特征分析  

Metabolic characteristics of Jiucaiping 2 tobacco leaves based on UPLC⁃HESI⁃MS/MS

作　　者：申树林 刘涛 邹焱 顾怀康 何轶 尹能文 孙夫军[1,2] 曹廷茂[3] 喻奇伟 陈秀娥 李成 李彩斌 曲存民 SHEN Shu-lin;LIU Tao;ZOU Yan;GU Huai-kang;HE Yi;YIN Neng-wen;SUN Fu-jun;CAO Ting-mao;YU Qi-wei;CHEN Xiu-e;LI Cheng;LI Cai-bin;QU Cun-min(College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China;Academy of Modern Agricultural Sciences,Southwest University,Chongqing 400716,China;Guizhou Province Tobacco Company Bijie City Company,Bijie,Guizhou 551700,China;Guizhou Academy of Tobacco Science,Guiyang 550000,China;China Tobacco Hunan Industry Co.Ltd.,Changsha 410019,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400716 [2]西南大学现代农业科学研究院,重庆400716 [3]贵州省烟草公司毕节市公司,贵州毕节551700 [4]贵州省烟草科学研究院,贵阳550000 [5]湖南中烟工业有限责任公司,长沙410019

出　　处：《西南农业学报》2023年第12期2678-2684,共7页Southwest China Journal of Agricultural Sciences

基　　金：贵州省烟草公司毕节市公司2021年科技项目(2021520500240048);重庆市博士后项目(cstc2020jcyj-bshX0016)。

摘　　要：【目的】探究毕节市烟草公司选育的地方烤烟品种韭菜坪2号与对照品种云烟87在毕节地区种植的代谢差异,为后续揭示韭菜坪2号烟叶香型风格特征的分子机理奠定基础,同时为该品种的推广应用提供理论依据。【方法】以云烟87为对照材料,采用液相色谱-质谱联用技术,对毕节市赫章县3个烟叶产区(达依、可乐、铁匠)的云烟87与韭菜坪2号新鲜烟叶进行代谢组分析。【结果】在烟叶样本中共检测到1880个离子峰,主成分分析表明3个烟叶产区的韭菜坪2号与云烟87均具有显著的代谢差异。以差异倍数大于2,t检验的P<0.05为标准进行差异代谢物筛选,各产区差异离子数量排序为达依>可乐>铁匠。同时鉴定到46个离子峰在3个产区的韭菜坪2号中积累量升高,49个离子峰在3个产区的韭菜坪2号中积累量降低。进一步在韭菜坪2号积累量升高的离子中鉴定到1种类黄酮代谢物M_9637[木犀草素7-(6’-丙二酰糖苷)]。对2个烟叶品种进行酚酸、类黄酮的广泛靶向代谢分析发现,两种类黄酮物质(异属李素和槲皮素)在韭菜坪2号中具有积累量升高的趋势。对降烟碱与尼古丁的分析发现,两种代谢物在韭菜坪2号中均表现出积累量降低的趋势。【结论】对毕节市赫章县3个烟叶产区的韭菜坪2号和云烟87新鲜烟叶代谢组比较分析表明,韭菜坪2号烟叶中类黄酮代谢水平高于云烟87,烟碱类物质代谢水平低于云烟87。上述两类物质的积累差异可能是韭菜坪2号烟叶香型风格特征形成的基础。【Objective】The present paper aimed to explore the metabolic differences between the local flue⁃cured tobacco variety Jiucaiping 2 selected by the Bijie Tobacco Company and the control variety Yunyan 87 planted in the Bijie area,lay the foundation for further revealing the molecular mechanism of the aroma style characteristics of Jiucaiping 2 tobacco leaves,and provide theoretical basis for the promotion and application of this variety.【Method】Taking Yunyan 87 as the reference material,the metabolome analysis of Yunyan 87 and Jiucaiping 2 fresh tobacco leaves from three tobacco leaf production areas(Dayi,Kele,Tiejiang)in Hezhang county,Bijie city was carried out by liquid chromatography⁃mass spectrometry.【Result】A total of 1880 peaks were detected in the tobacco samples,and principal component analysis showed significant metabolic differences between Jiucaiping 2 and Yunyan 87 in three tobacco producing regions.Metabolite difference analy⁃sis was performed with difference multiple greater than 2 and the P<0.05 of t⁃test as the standard.The order of ion quantity differences a⁃mong different production areas was Dayi>Kele>Tiejiang.At the same time,it was identified that 46 ion peaks had an increased accumula⁃tion in Jiucaiping 2 from three production areas,while 49 ion peaks had a decreased accumulation in Jiucaiping 2 from three production are⁃as.A flavonoid metabolite M_9637[luteolin 7⁃(6′⁃malonylglucoside)]was further identified among the peaks with increased accumulation in Jiucaiping 2.Widely⁃targeted metabolic analysis of phenolic acids and flavonoids in two tobacco varieties revealed an increasing trend of ac⁃cumulation of two types of flavonoids(isorhamnetin and quercetin)in Jiucaiping 2.The analysis of nornicotine and nicotine showed that both metabolites tended to accumulate and decrease in Jiucaiping 2.【Conclusion】In the study,the comparative analysis of the metabolites of Ji⁃ucaiping 2 and Yunyan 87 in three tobacco producing areas found that the metabolism of fl

关 键 词：韭菜坪2号 烟叶 代谢组 次生代谢物 类黄酮 尼古丁 

分 类 号：S572[农业科学—烟草工业]