绿脓菌素产量优化及其降解含氮化合物  

Yield optimization of pyocyanin and its degradation of nitrogenous compounds

作　　者：马思嘉 刘玉香[1] MA Sijia;LIU Yuxiang(College of Environmental Science and Engineering,Taiyuan University of Technology,Taiyuan 030024,Shanxi Province,China)

机构地区：[1]太原理工大学环境科学与工程学院,山西太原030024

出　　处：《化学工程》2024年第2期23-28,60,共7页Chemical Engineering(China)

基　　金：山西省基础研究计划项目(202103021224106)。

摘　　要：为探究细菌胞外分泌物对含氮化合物降解能力的影响,以铜绿假单胞菌(Pseudomonas aeruginosa)产生的PYO(绿脓菌素)为研究对象,进行单因素试验及运用RSM(响应曲面法)的统计学模型以优化PYO产量,电化学测试探讨PYO降解的影响机理,并将其用于含氮化合物的降解。结果表明:对PYO生成有显著影响的3种因素有C/N元素质量比、pH值和Mg^(2+)质量浓度,经过方差分析和评估得到最佳条件:C/N比为24,pH值为7.9,Mg^(2+)质量浓度0.96 g/L,此时PYO产量为10.74 mg/L。电化学测试说明降解率的提升得益于PYO的氧化还原性。将PYO用于含氮化合物中,TOC(总有机碳)和TN(总氮)降解率达到75%、92%,说明PYO可促进吲哚、吡啶的降解,可为PYO用于废水处理研究提供参考。In order to explore the influence of bacterial extracellular secretion on the degradation ability of nitrogenous compounds,PYO produced by Pseudomonas aeruginosa was taken as the research object.A single factor experiment and a statistical model of RSM(response surface method)were carried out to optimize the PYO.The effect mechanism of PYO on the degradation was discussed by electrochemical test,and PYO was applied to the degradation nitrogenous compounds.The results show that three factors significantly affecting PYO are C/N mass ratio,pH and Mg^(2+)mass concentration.Through the analysis of variance and evaluation,the optimal value of C/N ratio,pH and Mg^(2+)mass concentration are 24,7.9 and 0.96 g/L,respectively,and the output of PYO is 10.74 mg/L.Electrochemical test indicates that the improvement of degradation rates is due to the strong oxidation-reducing ability of PYO.When PYO is used in nitrogenous compounds,the TOC and TN removal rate reach 72%and 92%,indicating that PYO can promote the degradation of indole and pyridine.A reference can be provided for the study of PYO for wastewater treatment.

关 键 词：铜绿假单胞菌 胞外分泌物 绿脓菌素 响应曲面 含氮化合物 

分 类 号：X172[环境科学与工程—环境科学]