联合检测EB病毒不同抗体及EB病毒DNA在鼻咽癌血清学诊断中的价值探讨  

Exploration on value of combined detection of different antibodies against EB virus and EB virus DNA in serological diagnosis of nasopharyngeal carcinoma

作　　者：周梓婷 何雨[1] 潘康健 郑智弦 Zhou Ziting;He Yu;Pan Kangjian;Zheng Zhixian(Department of Clinical Laboratory,the First Affiliated Hospital of Guangxi Medical University&Key Laboratory of Clinical Laboratory Diagnostics of Guangxi Colleges,Nanning,Guangxi 530021,China;Wuzhou Red Cross Hospital,Wuzhou,Guangxi 543099,China)

机构地区：[1]广西医科大学第一附属医院检验科/广西高校临床检验诊断学重点实验室,广西南宁530021 [2]梧州市红十字会医院,广西梧州543099

出　　处：《齐齐哈尔医学院学报》2023年第21期2041-2044,共4页Journal of Qiqihar Medical University

基　　金：2021年度广西高校中青年教师科研基础能力提升项目(2021KY1951)。

摘　　要：目的 探讨EB病毒不同抗体与EB病毒DNA联合检测鼻咽癌的诊断价值。方法 选择2018年4月—2022年6月本院收治的200例鼻咽癌患者为观察组,同期选择156名健康体检者为对照组,均实施EB病毒不同抗体、EB病毒DNA检测,以病理检查结果为金标准,汇总两组检测结果、不同分期者检测结果,统计各个指标阳性检出率及诊断效能,分析联合与单一诊断价值。结果 观察组Rta-IgG抗体、EBV-DNA与对照组比差异显著,且Ⅳ期Rta-IgG抗体、EBV-DNA高于Ⅰ期、Ⅱ期、Ⅲ期(P<0.05);观察组患者血清中三种抗体为阳性,而对照组则相反,大部分为阴性(P<0.05);以病理检查结果为金标准,VCA-IgA抗体+Rta-IgG抗体+EA-IgA抗体+EBV-DNA阳性率高于EB病毒抗体阳性率、EB病毒DNA阳性率(P<0.05);VCA-IgA抗体+Rta-IgG抗体+EA-IgA抗体+EBV-DNA检测准确度高于其他检测方案(P<0.05)。结论 在鼻咽癌诊断中应用EB病毒不同抗体及EB病毒DNA联合方案可提高诊断准确率,为临床疾病的诊断及分期提供参考,值得借鉴及实施。Objective To explore the value of different antibodies against EB virus and EB virus DNA combining detection for the diagnosis of nasopharyngeal carcinoma.Methods 200 nasopharyngeal carcinoma patients who were admitted to our hospital from April 2018 to June 2022 were enrolled in the observation group,156 healthy individuals were selected as the control group during the same period,and EB virus antibodies and EB virus DNA tests were performed among an the subjects.The pathological examination results were used as the gold standard to summarize the test results of two groups and different stages.The positive detection rate and diagnostic efficacy of each indicator were calculated,and the combined and single diagnostic values were analyzed.Results There were significant differences in Rta-IgG antibodies and EBV-DNA between the observation group and the control group,and the levels of Rta-IgG antibodies and EBV-DNA in stageⅣwere higher than those in stagesⅠ,Ⅱ,andⅢ(P<0.05).The three antibodies in the serum of the observation group patients were positive,while the control group was the opposite,with most of them being negative(P<0.05).Using pathological examination results as the gold standard,the positive rate of VCA IgA antibody+Rta IgG antibody+EA IgA antibody+EBV-DNA was higher than that of EB virus antibody and EB virus DNA(P<0.05).The accuracy of VCA-IgA antibody+Rta-IgG antibody+EA-IgA antibody+EBV-DNA detection was higher than other detection schemes(P<0.05).Conclusions The application of EB virus antibodies and EB virus DNA combination protocol in the diagnosis of nasopharyngeal carcinoma can improve diagnostic accuracy,provide reference for clinical disease diagnosis and staging,and is worth learning from and implementing.

关 键 词：EB病毒 EB病毒DNA 鼻咽癌 血清学 诊断 EA-IgA抗体 

分 类 号：R739.63[医药卫生—肿瘤]