基于16S rDNA分析首发抑郁症患者的代谢紊乱因素  

Analysis of metabolic disorders in patients with first-episode depression based on 16S rDNA.

作　　者：辛红梅[1] 王双喜 李丽[1] 威力斯 齐玉梅 张婧 王无忌 XIN Hongmei;WANG Shuangxi;LI Li(Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010110 [2]内蒙古师范大学心理学院 [3]内蒙古自治区第三医院

出　　处：《精神医学杂志》2023年第2期161-166,共6页Journal of Psychiatry

基　　金：内蒙古自治区高等学校科学研究项目(编号:NJZY22661);内蒙古自治区卫生健康科技计划项目(编号:202202144);内蒙古医科大学面上项目(编号:YKD2022MS078,YKD2022MS074)。

摘　　要：目的通过16S rDNA测序技术对首发抑郁症患者肠道菌群分布、多样性和基因功能进行分析,探讨抑郁症患者代谢变化的情况。方法选取首发抑郁症患者13例为研究组,选取本市健康对照11名为对照组。采用16S rDNA基因测序法对两组肠道菌群进行物种注释分析和功能比较,统计两组肠道菌群分布、多样性和基因功能的变化。结果PCoA分析显示,两组群落组成结构比较,差异有统计学意义(P<0.05)。在门水平,研究组厚壁菌门(Fimicutes)降低、拟杆菌门(Bacteroidota)升高(P<0.05)。在属水平,研究组拟杆菌属(Bacteroides)和副拟杆菌属(Parabacteroides)的丰度升高(P<0.05)。研究组标志微生物为:变形菌纲(Alphaproteobacteria),拟杆菌门(Bacteroidota),拟杆菌纲(Bacteroidia)和拟杆菌目(Bacteroidales)。在三级KEGG途径分类下,与对照组相比,研究组苯丙氨酸生物合成、色氨酸生物合成、半胱氨酸和蛋氨酸代谢、缬氨酸合成、亮氨酸和异亮氨酸合成、胰岛素抗性和胰岛素信号路径的基因表达量降低(P<0.05),转运功能、缬氨酸降解、亮氨酸和异亮氨酸降解、脂肪酸降解、谷胱甘肽代谢和PPAR信号路径的基因表达量升高(P<0.05)。结论肠道菌群结构及其基因功能的改变是导致机体代谢紊乱的重要因素,检测肠道菌群变化可为临床防治抑郁症提供新思路,依据基因功能变化可为研究抑郁症发生的具体机制提供参考。Objective To explore the distribution,diversity and gene function of intestinal flora in patients with first-episode depression by 16S rDNA sequencing technology,and to explore the metabolic changes in patients with depression.Methods A total of 13 patients with first-episode depression were selected as study group,and 11 healthy controls were selected as control group.16S rDNA gene sequencing method was used to analyze the species annotation analysis and function comparison of the intestinal flora of patients,and the changes in the distribution,diversity and gene function of the intestinal flora of the two groups were analyzed.Results PCoA analysis showed that there existed significant difference in the community composition structure between two groups(P<0.05).At the phylum level,Fimicutes was significantly decreased and Bacteroidota was significantly increased in study group(P<0.05).At the genus level,the abundances of Bacteroides and Parabacteroides in study group were significantly increased(P<0.05).The marker microorganisms in study group were Alphaproteobacteria,Bacteroidota,Bacteroidia and Bacteroidales.Under the three-level KEGG pathway classification,the expression levels of genes in phenylalanine biosynthesis,tryptophan biosynthesis,cysteine and methionine metabolism,valine synthesis,leucine and isoleucine synthesis,insulin resistance and insulin signaling pathways were significantly decreased in study group compared with those in control group(P<0.05).The expression levels of genes in transport function,valine degradation,leucine and isoleucine degradation,fatty acid degradation,glutathione metabolism and PPAR signaling pathways were significantly increased in study group compared with those in control group(P<0.05).Conclusion The changes in intestinal flora structure and gene function are important factors leading to metabolic disorders.Detecting the changes in intestinal flora can provide new ideas for clinical prevention and treatment of depression,and provide a reference for studying the specific

关 键 词：肠道菌群 抑郁症 代谢紊乱 16S rDNA扩增子测序 

分 类 号：R749.4[医药卫生—神经病学与精神病学]