薏苡仁的化学成分及抗氧化活性研究  

Chemical Constituents and Antioxidant Activity of Coicis Semen

作　　者：朱蔚芊 殷鑫 汪祖华 周英 周永强 胡瑞航 俸婷婷 ZHU Weiqian;YIN Xin;WANG Zuhua;ZHOU Ying;ZHOU Yongqiang;HU Ruihang;FENG Tingting(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Research Center for Application and Development of Medicine and Food Dual-Use Resources,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]贵州中医药大学药食两用资源应用与开发研究中心,贵阳550025

出　　处：《中国药学杂志》2023年第22期2054-2061,共8页Chinese Pharmaceutical Journal

基　　金：贵州省科技厅基础研究计划项目资助(黔科合基础-ZK[2021]一般519);贵州省教育厅青年人才成长项目资助(黔教合KY字[2021]202号);贵州省“十四五”中医药、民族医药重点学科项目资助[QZYYZDXK(JS)-2021-03];贵州中医药大学本科教学工程建设项目资助(贵中医教学工程合字[2019]54号);贵州中医药大学博士启动基金项目资助(贵中医博士启动[2019]11号)。

摘　　要：目的研究薏苡仁的化学成分和抗氧化活性。方法运用硅胶柱色谱、ODS柱色谱以及半制备型高效液相色谱等技术进行分离纯化,并根据波谱数据鉴定化合物的结构;以维生素C为阳性药,采用1,1-二苯基-2-苦基肼(DPPH)和2,2′-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)自由基清除法测定部分苯丙素类化合物的抗氧化活性。结果从薏苡仁的乙酸乙酯和正丁醇部位中分离得到24个单体化合物,分别为鉴定为4-(3-hydroxy-propenyl)-phenol(1)、salidrosideⅠ(2)、对香豆酸(3)、阿魏酸(4)、1-O-阿魏酰甘油酯(5)、linusitamarin(6)、americanol A(7)、isoamericanol A(8)、9′-O-methylisoamericanol A(9)、3,3′-bisdemethylpinoresinol(10)、icariol A2(11)、圣草酚(12)、5,7-二羟基色原酮-5-O-β-D-葡萄糖苷(13)、5,7-二羟基色原酮-7-O-芸香糖苷(14)、1,2-heptanediol(15)、1,2,3-octanetriol(16)、3-hydroxyoctanoic acid(17)、9-hydroxynonanoic acid(18)、(+)-(4S)-(2E)-4-羟基-2-壬烯酸(19)、9-hydroxy-10-undecenoic acid(20)、8-hydroxyoctanoic acid methyl ester(21)、己烷酸单甘油酯(22)、trans-N-p-coumaroyl tyramine(23)、对羟基苯甲醇(24)。结论化合物1~2、5~11、13~22、24为首次从薏苡属中分离鉴定得到,化合物4、7~11表现出不同程度的抗氧化活性,其中化合物7、8、10的抗氧化作用较好。OBJECTIVE To study the chemical constituents and antioxidant activity of the seeds of Coicis Semen.METHODS Silica gel column chromatography,ODS column chromatography and semi-preparative high performance liquid chromatography were used to isolate and purify the compounds,and the structures of the compounds were identified according to the spectral data.The antioxidant activities of some phenylpropanoid compounds were determined by DPPH and ABTS free radical scavenging METHODS with vitamin C as positive drug.RESULTS Twenty-four monomer compounds were isolated from the ethyl acetate and n-butanol fractions of the seeds of Coicis Semen,which were identified as 4-(3-hydroxypropenyl)-phenol(1)、salidrosideⅠ(2),p-coumaric acid(3),ferulic acid(4),1-O-feruloylglycerol(5),linusitamarin(6),americanol A(7),isoamericanol A(8),9′-O-methylisoamericanol A(9),3,3′-bisdemethylpinoresinol(10),icariol A2(11),eriodictyol(12),5-β-D-glucosyloxy-7-hydroxychromone(13),5,7-dihydroxychromone-7-O-rutinoside(14),1,2-heptanediol(15),1,2,3-octanetriol(16),3-hydroxyoctanoic acid(17),9-hydroxynonanoic acid(18),(+)-(4S)-(2E)-4-hydroxy-2-nonenoic acid(19),9-hydroxy-10-undecenoic acid(20),8-hydroxyoctanoic acid methyl ester(21),monoglyceryl hexanoate(22),trans-N-p-coumaroyl tyramine(23)and p-hydroxybenzyl alcohol(24).CONCLUSION Compounds 1-2,5-11,13-22 and 24 are isolated and identified from Coix for the first time,compounds 4 and 7-11 show different degrees of antioxidant activity,among which compounds 7,8 and 10 have better antioxidant effect.

关 键 词：薏苡仁 苯丙素 抗氧化 1 1-二苯基-2-苦基肼 2 2′-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐 

分 类 号：R284[医药卫生—中药学]