ncRNA介导的EphB4上调与低级别胶质瘤不良预后及肿瘤免疫浸润的相关性  

Correlation of ncRNA-mediated Up-regulation of EphB4 with Poor Prognosis and Tumor Immunoinfiltration in Low-grade Glioma

作　　者：吴铮 任志伟[1] 高润石 孙轲 张国君 WU Zheng;REN Zhiwei;GAO Runshi(Department of Functional Neurosurgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院功能神经外科,北京100053 [2]国家儿童医学中心、首都医科大学附属北京儿童医院功能神经外科,100045

出　　处：《医学研究杂志》2024年第2期143-150,177,共9页Journal of Medical Research

摘　　要：目的探讨促红细胞生成素生成人肝细胞受体B4(erythropoietin-producing human hepatocellular receptors B4,EphB4)在低级别胶质瘤(lower-grade glioma,LGG)组织中的表达、其上游靶点以及与患者预后及肿瘤免疫浸润的相关性,分析其作为治疗靶点的潜在作用。方法首先应用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库胶质瘤数据分析EphB4在胶质瘤与正常脑组织中的表达差异,并用基因表达谱交互分析(Gene Expression Profiling Interactive Analysis,GEPIA)数据库分析EphB4对不同癌症生存期的影响。用R语言和starBase数据库分析EphB4可能上游调节非编码RNA(non-coding RNA,ncRNA)。用肿瘤免疫评估资源(Tumor Immune Estimation Resource,TIMER)数据库进行EphB4表达与肿瘤免疫细胞浸润、免疫细胞生物学标志物和免疫检查点相关性分析。结果UBA6-AS1/hsa-miR-346轴是最有潜力影响LGG中EphB4表达的上游ncRNA相关通路。同时,EphB4水平与LGG肿瘤免疫细胞浸润、免疫细胞生物学标志物、免疫检查点表达均呈显著正相关。结论ncRNA介导的EphB4上调与LGG的不良预后和肿瘤免疫浸润相关。Objective To investigate the expression of erythropoietin-producing human hepatocellular receptors B4(EphB4)and its upstream targets as well as correlation with prognosis in low-grade glioma(LGG),for analyzing its potential role as a therapeutic target.Methods Firstly analyzed the expression of EphB4 in glioma and normal brain tissue by The Cancer Genome Atlas(TCGA)database.Then,Gene Expression Profiling Interactive Analysis(GEPIA)database was used to analyze the effects of EphB4 on various cancer survival.The possible upstream regulatory non-coding RNA(ncRNA)of EphB4 were analyzed by R and starBase database.The correlation of EphB4 with tumor immune cell infiltration,biomarkers of immune cells and immune checkpoint expression were analyzed using the Tumor Immune Estimation Resource(TIMER)database.Results UBA6-AS1/hsa-miR-346 axis was the most potential upstream non-coding RNA(ncRNA)related pathway to influence expression of EphB4 in LGG.At the same time,the level of EphB4 was positively correlated with the tumor immune cell infiltration,immune cell biomarkers and immune checkpoint in LGG.Conclusion NcRNA-mediated up-regulation of EphB4 is associated with poor prognosis and tumor immunoinfiltration in LGG.

关 键 词：低级别胶质瘤 促红细胞生成素生成人肝细胞受体B4 非编码RNA 免疫浸润 不良预后 

分 类 号：R739.41[医药卫生—肿瘤]