miR-652-3p通过靶向Nptn抑制胶质母细胞瘤的增殖和迁移  

miR-652-3p suppresses glioblastoma proliferation and migration by targeting Nptn

作　　者：胥敏[1] 陈正楼[1] 王云江 火旭其 张新化 王宏盛[1] XU Min;CHEN Zhenglou;WANG Yunjiang(Department of Neurosurgery,Yancheng Third People s Hospital,The Sixth Affiliated Hospital of Nantong University,Yancheng School of Clinical Medicine of Nanjing Medical University,Yancheng 224002,China)

机构地区：[1]盐城市第三人民医院神经外科,南通大学第六附属医院,南京医科大学盐城临床医学院,盐城224002

出　　处：《临床神经外科杂志》2024年第1期43-48,53,共7页Journal of Clinical Neurosurgery

基　　金：国家自然科学基金资助项目(31171038);江苏医药职业学院临床学院专项科研发展基金课题项目(20219103,20229106)。

摘　　要：目的 探讨miR-652-3p对胶质母细胞瘤(GAM)增殖和迁移的影响。方法 用miR-652-3p模拟物转染胶质母细胞系C6和U87,然后分别通过CCK8、细胞流式、EdU、Transwell和划痕实验研究miR-652-3p对细胞增殖和迁移的影响。利用实时定量聚合酶链反应(qRT-PCR)、蛋白质免疫印迹(Western Blot)和双荧光素酶报告基因实验检测miR-652-3p与Neuroplastin(Nptn)的互作关系。通过CCK8、细胞流式、EdU、Transwell和划痕实验研究Nptn对细胞增殖和迁移的影响。结果 miR-652-3p的过表达可以抑制C6和U87细胞的增殖和迁移;双荧光素酶报告基因实验结果表明miR-652-3p可以与Nptn结合,Nptn表达水平受到miR-652-3p水平的影响;干扰Nptn的表达可以抑制C6和U87细胞的增殖和迁移。结论 miR-652-3p通过靶向Nptn抑制GBM的增殖和迁移。Objective To investigate the effect of miR-652-3p on glioblastoma(GBM)cell lines.Methods GBM C6 and U87 cell lines were transfected with miR-652-3p mimic,and then the effects of miR-652-3p on cell proliferation and migration were detected by CCK8,flow cytometry,EdU,Transwell and wound-healing assays,respectively.The interaction between miR-652-3p and neuroplastin(Nptn)was detected by quantitative real-time polymerase chain reaction(qRT-PCR),Western blot and dual luciferase reporter gene assays.The effects of Nptn on cell proliferation and migration were investigated by CCK8,cytometry,EdU,Transwell and wound-healing assays.Results Overexpression of miR-652-3p inhibits the proliferation and migration of GBM cells.Nptn is a functional target of miR-652-3p.Interfering with the expression of Nptn inhibits the proliferation and migration of GBM cells.Conclusion miR-652-3p suppresses GBM proliferation and migration by targeting Nptn.

关 键 词：miR-652-3p Nptn 胶质母细胞瘤 增殖 迁移 

分 类 号：R739.41[医药卫生—肿瘤]