VNN1在溃疡性结肠炎中表达的研究  

Expression of VNN1 in Ulcerative Colitis

作　　者：布娅·米然别克 王秋玲 李得阳 艾合买江·库尔班江[1] 高峰[1] Buya·MIRANBIEKE;WANG Qiuling;LI Deyang;Aihemaijiang·KUERBANJIANG;GAO Feng(Department of Gastroenterology,the People's Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Clinical Research Center for Digestive Diseases,Urumqi,830001)

机构地区：[1]新疆维吾尔自治区人民医院消化内科新疆消化系统疾病临床研究中心,830001

出　　处：《胃肠病学》2023年第5期264-268,共5页Chinese Journal of Gastroenterology

基　　金：新疆维吾尔自治区自然科学基金(2019D01C129)。

摘　　要：目前临床上仍缺乏与溃疡性结肠炎(UC)疾病进展和预后相关的分子标志物。目的:探讨UC患者肠黏膜组织、血清和粪便中泛酰巯基乙胺酶1(VNN1)表达及其临床意义。方法:选择2018年12月—2020年1月新疆维吾尔自治区人民医院治疗的UC患者100例和健康志愿者100名,留取结肠镜活检组织、血液和粪便标本。应用PCR、蛋白质印迹法和免疫组化法分别检测肠黏膜组织中VNN1 mRNA和蛋白表达,ELISA法测定血清和粪便中VNN1表达。结果:UC患者肠黏膜组织中VNN1 mRNA和蛋白表达均明显高于健康对照者(P<0.05)。免疫组化法显示UC患者肠黏膜组织中VNN1表达明显高于健康对照组(P<0.05)。ELISA法显示UC患者血清VNN1表达明显高于健康对照者(P<0.05),而粪便中VNN1表达无明显差异(P>0.05)。结论:UC患者肠黏膜组织和血液中VNN1高表达,可作为UC新的分子标志物。Background:Molecular markers associated with disease progression and prognosis of ulcerative colitis(UC)are still lacking in clinic.Aims:To investigate the expression and clinical significance of vanin 1(VNN1)in intestinal mucosal tissue,serum and stool in patients with UC.Methods:A total of 100 UC patients and 100 healthy volunteers from Dec.2018 to Jan.2020 at the People's Hospital of Xinjiang Uygur Autonomous Region were recruited,and colonoscopy biopsy tissue samples,blood samples and stool samples were collected.PCR,Western blotting and immunohistochemistry were used to detect mRNA and protein expressions of VNN1 in intestinal mucosal tissue,respectively.The expression of VNN1 in serum and stool was determined by ELISA.Results:The mRNA and protein expressions of VNN1 in intestinal mucosal tissue in patients with UC were significantly higher than those in healthy controls(P<0.05).Immunohistochemistry results showed that VNN1 expression was significantly higher in intestinal mucosal tissue in patients with UC than in healthy controls(P<0.05).ELISA results showed that serum expression of VNN1 in patients with UC was significantly higher than that in healthy controls(P<0.05).There was no significant difference in expression of VNN1 in stool between patients with UC and healthy controls(P>0.05).Conclusions:The expressions of VNN1 in intestinal mucosal tissue and blood in UC patients are high,and can be used as a molecular marker of UC.

关 键 词：结肠炎 溃疡性 泛酰巯基乙胺酶1 生物学标记 

分 类 号：R574.62[医药卫生—消化系统]