miR-143-3p通过靶向integrinβ1抑制肝癌进展  

miR-143-3p inhibits liver cancer progression by targeting integrinβ1

作　　者：李丽坤[1] 邸雅南[1] 陈帝[1] 张晶[1] Li Likun;Di Yanan;Chen Di;Zhang Jing(Department of Gastroenterology,Beijing Aerospace General Hospital,Beijing 100076,China)

机构地区：[1]北京航天总医院消化内科,北京100076

出　　处：《中国组织化学与细胞化学杂志》2023年第5期480-488,共9页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探讨肝癌中miR-143-3p的表达及其对肝癌细胞恶性生物学行为的影响,并分析潜在的机制。方法收集肝癌组织,用RT-qPCR和Western blot法分别检测miR-143-3p和integrinβ1的表达。体外培养肝癌细胞,并用miR-143-3p mimics转染后,用XTT和EDU法检测过表达miR-143-3p对细胞增殖活力的影响,用Transwell法检测过表达miR-143-3p对细胞迁移与侵袭的影响,Western blot法检测过表达miR-143-3p对integrinβ1表达的影响。用荧光素酶活性法检测miR-143-3p与integrinβ1的靶向关系。用XTT、EdU和Transwell法检测过表达integrinβ1对已转染miR-143-3p mimics的肝癌细胞增殖、迁移与侵袭的影响。结果与癌旁正常组织比较,肝癌组织中miR-143-3p表达降低,integrinβ1表达升高,且二者均随疾病分期进展进一步降低或增高。过表达miR-143-3p能抑制肝癌细胞增殖、迁移和侵袭,并能下调integrinβ1表达。过表达integrinβ1能逆转miR-143-3p对肝癌细胞的抑制作用。integrinβ1为miR-143-3p的靶点。结论miR-143-3p在肝癌中表达下调,而上调miR-143-3p能通过靶向integrinβ1抑制肝癌细胞增殖、迁移与侵袭。Objective To investigate the expression of miR-143-3p in liver cancer and its effect on malignant biological behavior of liver cancer cells,and to analyze the potential mechanism.Methods Liver cancer tissues were collected and the expression of miR-143-3p and integrinβ1 were detected by RT-qPCR and Western blot,respectively.Liver cancer cells were cultured in vitro and then transfected with miR-143-3p mimics.The effect of miR-143-3p expression on cell proliferation viability was detected by XTT and EdU assays,and the effect of miR-143-3p expression on cell migration and invasion was detected by Transwell assay.The influence of miR-143-3p expression on integrinβ1 expression was detected by Western blot.The targeting relationship between miR-143-3p and integrinβ1 was detected by luciferase activity assay.The effects of integrinβ1 over-expression on the proliferation,migration and invasion of liver cancer cells transfected with miR-143-3p mimics were detected by XTT,EdU and transwell assays.Results Compared with paracancer normal tissues,the expression of miR-143-3p and integrinβ1 was decreased and increased in liver cancer tissues,respectively,and both were further decreased or increased with disease stage progression.Over-expression of miR-143-3p inhibited the proliferation,migration and invasion of liver cancer cells,and down-regulated integrinβ1 expression.Over-expression of integrinβ1 reversed the inhibitory effect of miR-143-3p on liver cancer cells.Integrinβ1 was the target of miR-143-3p.Conclusion The expression of miR-143-3p is down-regulated in liver cancer,while up-regulation of miR-143-3p can inhibit the proliferation,migration and invasion of liver cancer cells by targeting integrinβ1.

关 键 词：miR-143-3p 肝癌 INTEGRINΒ1 增殖 迁移 侵袭 

分 类 号：R365[医药卫生—病理学]