细胞焦亡在急性脑梗死缺血再灌注损伤中的作用研究  

Role of Pyroptosis in Ischemia-reperfusion Injury in Acute Cerebral Infarction

作　　者：云强 刘雅虹 海俊杰 朱海波 江名芳[3] YUN Qiang;LIU Yahong;HAI Junjie;ZHU Haibo;JIANG Mingfang(Shenzhen Longhua District Central Hospital,Shenzhen 518110 China;Inner Mongolia Medical University Graduate School,Hohhot 010058 China;Department of Neurology Inner Mongolia Medical University Affiliated Hospital,Hohhot 010050 China)

机构地区：[1]深圳市龙华区中心医院,深圳518110 [2]内蒙古医科大学研究生院,呼和浩特010058 [3]内蒙古医科大学附属医院神经内科,呼和浩特010050

出　　处：《内蒙古医学杂志》2023年第12期1426-1431,共6页Inner Mongolia Medical Journal

基　　金：内蒙古自治区自然科学基金面上项目(编号:2020MS08197);内蒙古自治区自然科学基金联合项目(编号:2018LH08012);内蒙古自治区卫生健康科技计划项目(编号:202201264);内蒙古医科大学青年创新基金项目(编号:YKD2020QNCX010)。

摘　　要：目的探讨细胞焦亡在急性脑梗死缺血再灌注损伤中的作用和机制。方法2020年12月至2021年3月将12只SD雄性大鼠随机分为实验组和假手术组,实验组采用改良Longa法制备急性脑缺血再灌注大鼠模型,假手术组只暴露颈内动脉不阻断血流;实验组缺血处理2 h后再灌注,3 h后两组同时进行免疫荧光和蛋白质印迹(Western blot,WB)检测,最后进行脑含水量测定,同时采用酶联免疫吸附(ELISA)检测血清中IL-18和IL-1β的含量。结果实验组大鼠缺血2 h后按Bederson方法评定神经功能缺损,结果显示6只大鼠均造模成功,红四氮唑染色(TTC)提示实验组大鼠脑梗死重量百分比显著高于假手术组(P<0.001);WB检测发现实验组NLRP3、GSDMD及Caspase-1表达与假手术组差异无统计学意义;免疫荧光显示两组GFAP、Nestin和NeuN的表达均差异无统计学意义,但模型4、5、6的GFAP的表达量高于对照组;实验组脑含水量明显高于假手术组(P<0.01);实验组血清中IL-1β、IL-18的含量显著高于假手术组(P<0.001)。结论缺血早期再灌注后血清炎症因子明显增高,但脑细胞并未发现明显细胞焦亡。Objective To investigate the role and mechanism of cell charring in acute cerebral infarction with ischemia reperfusion injury.Methods During the period from December 2020 to March 2021,12 male SD rats were randomly divided into an experimental group and a sham operation group.The experimental group adopted a modified longa method to establish a rat model of acute cerebral ischemia/reperfusion.The sham operation group only exposed the internal carotid artery without blocking blood flow;The experimental group was subjected to ischemia for 2 hours before reperfusion.After 3 hours,both groups were simultaneously tested with immunofluorescence and Western blot(WB).Finally,brain water content was measured,and serum IL-18 and IL-1βwere detected by enzyme linked immunosorbent assay(ELISA)Content.Results After 2 hours of ischemia in the experimental group,neurological deficits were evaluated using the Bederson method.The results showed that all 6 rats were successfully modeled.Red tetrazolium staining(TTC)suggested that the weight percentage of cerebral infarction in the experimental group was significantly higher than that in the sham operation group(P<0.001);WB assay showed that there was no significant difference in the expression of NLRP3,GSDMD,and Caspase-1 between the experimental group and the sham operation group;Immunofluorescence showed that there was no significant difference in the expression of GFAP,Nestin,and NeuN between the two groups,but the expression of GFAP in models 4,5,and 6 was higher than that in the control group;The brain water content in the experimental group was significantly higher than that in the sham operation group(P<0.01);Experimental group serum IL-1β、The content of IL-18 in the sham operation group was significantly higher than that in the sham operation group(P<0.001).Conclusion Serum inflammatory factors significantly increase after early ischemia reperfusion,but there is no significant cell death in brain cells.

关 键 词：细胞焦亡 急性脑梗死 缺血再灌注 

分 类 号：R743.33[医药卫生—神经病学与精神病学]