宫颈癌患者HPV感染现状及miR-let-7d-5p、miR-3607-3p、CIP2A的诊断效能  

Current status of HPV infection among cervical cancer patients and diagnostic efficiencies of miR-let-7d-5p,miR-3607-3p and CIP2A

作　　者：况漫 肖祖文 闫益芬 孙雪 KUANG Man;XIAO Zu-wen;YAN Yi-fen;SUN Xue(Renmin Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]十堰市人民医院(湖北医药学院附属人民医院)妇科,湖北十堰442000

出　　处：《中华医院感染学杂志》2024年第1期85-89,共5页Chinese Journal of Nosocomiology

基　　金：湖北省卫生健康委员会科研项目(WJ2019F014)。

摘　　要：目的研究宫颈癌患者人乳头瘤病毒(HPV)感染情况及微小核糖核酸(miR)-let-7d-5p、miR-3607-3p、蛋白磷酸酶2A癌性抑制因子(CIP2A)的诊断效能。方法选取2021年1月-2022年12月十堰市人民医院收治的124例宫颈病变患者作为研究对象,其中宫颈癌患者36例、宫颈鳞状上皮内病变(SIL)患者41例和宫颈炎患者47例,分别纳入A组、B组和C组,比较三组HPV表达情况,绘制受试者工作特征(ROC)曲线分析miR-let-7d-5p、miR-3607-3p、CIP2A对宫颈癌的诊断效能。结果A组HPV阳性表达率、HPV E6/E7信使核糖核酸(mRNA)高载量率及宫颈组织CIP2A表达水平均高于B组、C组,且B组高于C组(P<0.05),A组宫颈组织miR-let-7d-5p、miR-3607-3p表达水平均低于B组、C组,且B组低于C组(P<0.05);ROC曲线分析结果显示,miR-let-7d-5p、miR-3607-3p、CIP2A联合检测诊断宫颈癌的曲线下面积(AUC)、敏感度和特异度分别0.937、94.44%和85.23%。结论宫颈癌患者HPV阳性表达率、HPV E6/E7 mRNA高载量率较高,宫颈组织miRlet-7d-5p、miR-3607-3p、CIP2A表达异常,三者联合检测诊断宫颈癌的诊断效能较高。OBJECTIVE To investigate the prevalence of human papillomavirus(HPV)infection among the patients with cervical cancer and analyze the diagnostic efficiencies of microribonucleic acid(miR)-let-7d-5p,miR-3607-3p and cancerous inhibitor of proteinphosphatase 2A(CIP2A).METHODS Totally 124 patients with cervical lesions who were treated in Shiyan People's Hospital from Jan 2021 to Dec 2022 were recruited as the research subjects,36 of whom had cervical cancer and were assigned as the group A,41 had squamous intraepithelial lesion(SIL)and were assigned as the group B,and 47 had cervicitis and were assigned as the group C.The expression of HPV was compared among the three group.The efficiencies of miR-let-7d-5p,miR-3607-3p and CIP2A in diagnosis of cer-vical cancer were analyzed by means of receiver operating characteristic(ROC)curves.RESULTS The positive rate of expression of HPV,high-load expression rate of HPV E6/E7 messenger ribonucleic acid(mRNA)and ex-pression level of CIP2A in cervical tissues were higher in the group A than in the group B and the group C;the a-bove indexes of the group B were higher than those of the group C(P<0.05).The expression levels of cervical tissue miR-let-7d-5p and miR-3607-3p of the group A were lower than those of the group B and the group C,and the expression levels of above indexes of the group B were lower than those of the group C(P<0.05).The result of ROC curve analysis showed that the area under curve(AUC)of the joint detection of miR-let-7d-5p,miR-3607-3p and CIP2A was 0.937 in diagnosis of cervical cancer,with the sensitivity 94.44%,the specificity 85.23%.CONCLUSION The patients with cervical cancer show high positive rate of expression of HPV and high-load expression rate of HPV E6/E7 mRNA as well as the abnormal expressions of miR-let-7d-5p,miR-3607-3p and CIP2A in cervical tissues.The joint detection of the three indexes has high efficiency in diagnosis of cervical cancer.

关 键 词：宫颈癌 宫颈鳞状上皮内病变 宫颈炎 微小核糖核酸-let-7d-5p 微小核糖核酸-3607-3p 蛋白磷酸酶2A癌性抑制因子 人乳头瘤病毒 诊断效能 

分 类 号：R737.33[医药卫生—肿瘤]