Genetic diversity of the S-type small subunit ribosomal RNA gene of Plasmodium knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia  

作　　者：Eric Tzyy Jiann Chong Joveen Wan Fen Neoh Tiek Ying Lau Kek Heng Chua Yvonne Ai-Lian Lim Ping-Chin Lee 

机构地区：[1]Biotechnology Research Institute,Universiti Malaysia Sabah,Jalan UMS,88400 Kota Kinabalu,Sabah,Malaysia [2]Department of Biomedical Science,Faculty of Medicine Building,University of Malaya,50603 Kuala Lumpur,Malaysia [3]Centre of Excellence for Research in AIDS(CERiA),University of Malaya,50603 Kuala Lumpur,Malaysia [4]Faculty of Science and Natural Resources,Universiti Malaysia Sabah,Jalan UMS,88400 Kota Kinabalu,Sabah,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2024年第2期84-90,共7页亚太热带医药杂志（英文版）

基　　金：This study was supported by the Ministry of Higher Education,Malaysia(FRGS0322-SG-1/2013);Universiti Malaysia Sabah(GUG0521-2/2020).

摘　　要：Objective:To determine the genetic diversity of Plasmodium(P.)knowlesi isolates from Sabah,Malaysian Borneo and Peninsular Malaysia,targeting the S-type SSU rRNA gene and including aspects of natural selection and haplotype.Methods:Thirty-nine blood samples infected with P.knowlesi were collected in Sabah,Malaysian Borneo and Peninsular Malaysia.The S-type SSU rRNA gene was amplified using polymerase chain reaction,cloned into a vector,and sequenced.The natural selection and haplotype of the S-type SSU rRNA gene sequences were determined using DnaSP v6 and illustrated using NETWORK v10.This study's 39 S-type SSU rRNA sequences and eight sequences from the Genbank database were subjected to phylogenetic analysis using MEGA 11.Results:Overall,the phylogenetic analysis showed no evidence of a geographical cluster of P.knowlesi isolates from different areas in Malaysia based on the S-type SSU rRNA gene sequences.The S-type SSU rRNA gene sequences were relatively conserved and with a purifying effect.Haplotype sharing of the S-type SSU rRNA gene was observed between the P.knowlesi isolates in Sabah,Malaysian Borneo,but not between Sabah,Malaysian Borneo and Peninsular Malaysia.Conclusions:This study suggests that the S-type SSU rRNA gene of P.knowlesi isolates in Sabah,Malaysian Borneo,and Peninsular Malaysia has fewer polymorphic sites,representing the conservation of the gene.These features make the S-type SSU rRNA gene suitable for comparative studies,such as determining the evolutionary relationships and common ancestry among P.knowlesi species.

关 键 词：Plasmodium knowlesi S-type small subunit ribosomal RNA Genetic diversity Natural selection HAPLOTYPE 

分 类 号：R531.3[医药卫生—内科学] R181.3[医药卫生—临床医学]