New Antibacterial Dihydropyrones Induced by Co-Culture of Penicillium crustosum PRB-2 and Penicillium citrinum HDN11-186  

作　　者：YU Guihong ZHOU Luning WU Guangwei LI Dehai 

机构地区：[1]Shandong Province Key Laboratory of Applied Mycology,and Qingdao International Center on Microbes Utilizing Biogas,School of Life Sciences,Qingdao Agricultural University,Qingdao 266109,China [2]School of Medicine and Pharmacy,and Sanya Oceanographic Institution,Ocean University of China,Qingdao 266003,China [3]College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China [4]Laboratory for Marine Drugs and Bioproducts,Laoshan Laboratory,Qingdao 266237,China

出　　处：《Journal of Ocean University of China》2024年第1期216-220,共5页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.41806167);the High-Level Talents Research Fund of Qingdao Agricultural University(No.665/1120034);the NSFC-Shandong Joint Fund(No.U1906212);the Major Project of the 14th Five-Year Plan(No.2022QNLM030003-1);the Natural Science Foundation of Shandong Province(No.ZR2021ZD28);the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(No.2021CXLH0012);the Youth Innovation Plan of Shandong Province(No.2019KJM004).

摘　　要：Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).

关 键 词：CO-CULTURE Penicillium crustosum Penicillium citrinum dihydropyrones antibacterial activity 

分 类 号：Q939.9[生物学—微生物学]