Genome-Wide Characterization of the Hyaluronidase Gene Family and Their Potential Roles in Viviparous Black Rockfish(Sebastes schlegeli)  

作　　者：LIU Zhiying NIU Jingning ZHAO Xi LIU Huaxiang LI Zibin HE Yan QI Jie 

机构地区：[1]MOE Key Laboratory of Marine Genetics and Breeding,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [2]Key Laboratory of Tropical Aquatic Germplasm of Hainan Province,Sanya Oceanographic Institute,Ocean University of China,Sanya 572025,China

出　　处：《Journal of Ocean University of China》2024年第2期487-498,共12页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.32273133,31970492).

摘　　要：Black rockfish(Sebastes schlegelii)has evolved viviparous reproduction mode.Different from the oviparous fishes,the spermatozoa of black rockfish are stored on the surface of ovarian follicle for over four months until the egg mature in ovary,while the adaptive evolution of fertilization-related genes remains to be studied.In the present study,based on the genome and transcriptome information of black rockfish,a total of 10 hyaluronidase genes were identified by phylogenetic and sequence analyses,including hyal1,hyal2,hyal3,hyal4,hyal6 and spam1a,spam1b,spam1c and spam1d.The spam1 subfamilies,including spam1a,spam1b,spam1c and spam1d,were significantly expanded compared to other species.The adaptive evolution of hyaluronidase was further investigated by selection pressure analysis of branch model and branch site model.The results showed that only spam1 subfamily was positively selected with a large number of positive selection sites,and the evolution rate was significantly higher thanthose of other teleosts.Two positively selected sites,LYS-171 and GLY-164,were located in the neutral hyaluronidase activity domain,which was pivotal for SPAM1 to participate in enzymatic hydrolysis of the extracellular hyaluronic acid matrix and mediate acrosome reaction in mammals.Furthermore,spam1 was mainly expressed in spermatocytes,secondary spermatocytes,and spermatids of testis,whereas it was not found in metamorphic sperm and mature sperm based on the spatiotemporal expression analysis.All results indicated that spam1 might originate from the testis and might be an adaptation of viviparous trait in black rockfish.

关 键 词：adaptation of viviparous black rockfish(Sebastes schlegelii) HYALURONIDASE selective pressure analysis 

分 类 号：S917.4[农业科学—水产科学]