HPLC法测定蒙药材金露梅中没食子酸含量  

Determination of Gallic Acid in Mongolian Medicine Potentilla Fruticosa by HPLC

作　　者：郭凤英 闫凤杰 关金凤 莫日根高娃 于海云 GUO Fengying;YAN Fengjie;GUAN Jinfeng;Morigengaowa;YU Haiyun(Tongliao Market Testing and Inspection Center,Tongliao 028000,China)

机构地区：[1]通辽市市场检验检测中心,内蒙古通辽028000

出　　处：《中国民族民间医药》2024年第2期24-27,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立高效液相法测定金露梅中没食子酸含量。方法:采用Kromasil 100-5-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸(10∶90)为流动相,流速为1.0 mL/min,检测波长273 nm,柱温30℃,进样量为10μL。结果:没食子酸在3.44~137.52μg/mL范围内线性关系良好,相关系数r=1.000;平均回收率为99.4%(RSD%=1.588%);6批不同产地金露梅中没食子酸含量在3.156~11.537 mg/g之间。结论:所建立的高效液相法稳定可靠,可用于金露梅中没食子酸的含量测定。Objective To establish an high performance liquid chromatography method for determination of gallic acid in Mongolian medicine potentilla fruticosa.Methods The test was performed in Kromasil 100-5-C18(250 mm×4.6 mm,5μm)under the elution of methanol-0.1%phosphoric acid(10∶90).The flow rate was 1.0 ml/min and the wavelength was set as 273 nm.The column temperature was 30℃and the injection volume was 10μL.Result The liner ranges of gallic acid fell into 3.44-137.52μg/mL(r=1.000).The average recovery of gallic acid was 99.4%(RSD=1.588%).The determination of galllic acid in potentilla fruticosa from 6 places of origin fell into 3.156-11.537 mg/g.Conclusion The established method is simple,stable and reliable.It can be used for determination of gallic acid in Mongolian medicine potentilla fruticosa.

关 键 词：蒙药材 金露梅 没食子酸 高效液相 含量测定 

分 类 号：R284.1[医药卫生—中药学]