乳腺癌组织中环状RNA CDR1as的表达及临床意义  

Expression and clinical significance of circular RNA CDR1as in breast cancer

作　　者：石碧霞 陶爽 徐溪[2] SHI Bixia;TAO Shuang;XU Xi(Breast Surgery Department,Jiangsu University Affiliated Wujin Hospital/Wujin Clinical College of Xuzhou Medical University,Changzhou 213164,China;Burn Plastic Surgery Department,Jiangsu University Affiliated Wujin Hospital/Wujin Clinical College of Xuzhou Medical University,Changzhou 213000,China)

机构地区：[1]江苏大学附属武进医院/徐州医科大学武进临床学院乳腺外科,江苏常州213164 [2]江苏大学附属武进医院/徐州医科大学武进临床学院烧伤整形外科,江苏常州213164

出　　处：《中国肿瘤外科杂志》2024年第1期54-60,共7页Chinese Journal of Surgical Oncology

摘　　要：目的探讨环状RNA CDR1as在乳腺癌组织中的表达以及CDR1as在乳腺癌细胞中的调控作用,为乳腺癌治疗提供新靶点。方法选取江苏大学附属武进医院2022年6月至2023年6月收治的45例三阴性乳腺癌患者,采用实时定量聚合酶链反应(RT-qPCR)检测乳腺癌组织及其癌旁正常乳腺组织中CDR1as表达情况。购入人正常乳腺上皮MCF-10A细胞及乳腺癌细胞系MDA-MB-231、MDA-MB-468细胞,采用RT-qPCR检测CDR1as在乳腺正常细胞与癌细胞中的表达情况。通过体外实验验证CDR1as的环状特性,将特异性针对CDR1as的siRNA及过表达载体转入MDA-MB-231细胞,Ad-CDR1as为高表达组,shCDR1s为低表达组,Ad-CON/Si-NC为阴性对照组。并采用蛋白质印迹分析(Western-blot)法检测细胞PCNA蛋白表达水平,MTT法、CCK-8法检测细胞增殖能力的改变,Transwell实验、划痕实验检测乳腺癌细胞迁移、侵袭能力。结果CDR1as在三阴性乳腺癌患者中的表达在不同肿瘤大小、淋巴结转移、组织学分级及临床分期上差异有统计学意义。乳腺癌患者癌组织中CDR1as的表达高于癌旁正常组织。乳腺癌细胞系MDA-MB-231中CDR1as表达量高于MCF-10A细胞。敲低/过表达CDR1as后,CDR1as沉默后乳腺癌细胞的细胞增殖量明显减少,而CDR1as过表达促进细胞增殖,差异有统计学意义。Western-blot结果显示Ad-CDR1as组PCNA表达高于Ad-CON组,Transwell结果显示Ad-CDR1as组细胞侵袭量大于Ad-CON组,而沉默CDR1as后细胞侵袭量显著降低;划痕试验显示,Ad-CDR1as组细胞迁移能力高于Ad-CON组,而沉默CDR1as后细胞迁移能力显著减弱,差异均有统计学意义。结论CDR1as在三阴性乳腺癌患者癌组织中高表达,且与临床病理特征相关。CDR1as在三阴性乳腺癌细胞中高表达,并促进三阴性乳腺癌细胞的增殖、侵袭和迁移,CDR1as可能是治疗三阴性乳腺癌的潜在靶点。Objective To investigate the expression of circular RNA CDR1as in breast cancer tissues and the regulatory role of CDR1as in breast cancer cells,so as to provide a new target for the treatment of breast cancer.Methods Real time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of circrna CDR1as in 45 cases of triple negative breast cancer in our hospital.The expression of circrna CDR1as in breast cancer tissues and normal breast tissues adjacent to breast cancer was detected.Human normal breast epithelial MCF-10A cells,breast cancer cell line MDA-MB-231 cells and MDA-MB-468 cells were purchased.RT-qPCR was used to detect the expression of CDR1as in normal breast cells and cancer cells.The circular characteristics of CDR1as were verified by in vitro experiments.SiRNA and overexpression vector specific for CDR1as were transferred into MDA-MB-231 cells.Ad CDR1as was used as the high expression group,shCDR1s as the low expression group,and Ad CON/Si NC as the negative control group.MTT assay and CCK-8 assay were used to detect the change of cell proliferation ability.Transwell assay and scratch healing assay were used to detect the migration and invasion ability of breast cancer cells.Results The expression of circrna CDR1as in three negative breast cancer patients was significantly different in tumor size,lymph node metastasis,histological grade and clinical stage.The expression of circrna CDR1as in the cancer tissues of breast cancer patients was higher than that in the adjacent normal tissues.The expression of circrna CDR1as in breast cancer cell line was higher than that in MCF-10A cells.After knockdown/overexpression of CDR1as,the cell proliferation of breast cancer cells was significantly reduced after CDR1as silencing,and the overexpression of Cdr1as promoted cell proliferation.Western blot analysis showed that the expression of PCNA in Ad-CDR1as group was higher than that in Ad-CON group.Transwell analysis showed that the invasion of cells in Ad-CDR1as group was higher than that

关 键 词：环状RNA CDR1as 三阴性乳腺癌 增殖 侵袭 转移 

分 类 号：R737.9[医药卫生—肿瘤]