基于多指标评价的胆囊炎豚鼠模型的建立与优化  

Establishment and Optimization of Guinea Pigs Model for Cholecystitis Based on Multi-index Evaluation

作　　者：胡静燕 黄松丽 周小慧 魏胜利[2] 胡秀华 HU Jingyan;HUANG Songli;ZHOU Xiaohui(School of Life Sciences,Beijing University of Chinese Medicine,Beijing 102401,China)

机构地区：[1]北京中医药大学生命科学学院,102401 [2]北京中医药大学中药学院,102401

出　　处：《医学研究杂志》2023年第12期45-49,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(32070357);北京市科技计划项目(2020071520102)。

摘　　要：目的探讨胆囊炎豚鼠模型的建立及优化方法,为进一步优化实验设计和研究胆囊炎的发病、治疗及作用机制提供实验基础。方法采取盐酸林可霉素皮下注射的方式,建立胆囊炎豚鼠模型,通过观察豚鼠一般状态、胆囊B超、血常规指标和肝胆组织病理学改变,确定胆囊炎模型的稳定性及应用前景。结果一般状态观察发现,模型组豚鼠出现不同程度的饮食减少、体质量下降、活动减少、毛发疏松无光泽、精神萎靡或烦躁不安等。胆囊B超结果显示,与对照组豚鼠比较,造模剂量越高,豚鼠的胆囊壁增厚越明显(P<0.05);血常规指标检测发现,造模剂量越高,血液炎性指标白细胞计数(white blood cell count,WBC)、淋巴细胞计数(lymphocyte,LYM)、中性粒细胞计数(neutrophil,NEUT)、单核细胞计数(monocyte,MONO)上升越显著(P<0.05);苏木精-伊红染色结果显示,造模剂量越高,胆囊和肝脏组织结构病变、炎性细胞浸润、肝细胞变性坏死等病理变化更加明显。结论本研究通过影像学指标结合血液及病理指标,多因素综合评价胆囊炎造模效果以及炎症模型的稳定性,较常规的胆囊病理分析法或结合血清炎性细胞因子酶联免疫吸附试验检测法更为直观、精确且廉价,且为药物治疗效果评价提供科学有效的检测指标;同时,确定豚鼠胆囊炎造模剂量为盐酸林可霉素120mg/kg的模型最为稳定,且不易自愈。Objective To investigate the establishment and optimization of guinea pigs model of cholecystitis,so as to provide the basis for further optimization of experimental design and research on the pathogenesis,treatment and mechanism of cholecystitis.Methods The guinea pigs model of cholecystitis was established by subcutaneous injection of lincomycin hydrochloride,the general condition,B-ultrasound of the gallbladder,blood routine indicators and hepatobiliary histology of the guinea pigs were observed to determine the stability and application prospect of the cholecystitis model.Results The general state observation showed that guinea pigs in the model group exhibited varying degrees of diet decrease,body weight loss,activity decrease,loose and lusterless hair,listlessness or irritability.B-ultrasound results showed that,compared with the control group of guinea pigs,with the higher modeling dose,the thickness of the gallbladder wall were more obvious(P<0.05).The blood routine index detection found that,with the higher modeling dose,the blood inflammatory indicators such as white blood cell count(WBC),lymphocyte(LYM),neutrophil(NEUT),monocyte(MONO)were increased significantly(P<0.05).The results of hematoxylin-eosin staining showed that,with the higher modeling dose,pathological changes such as structural changes of gallbladder and liver tissue structure,inflammatory cell infiltration,degeneration and necrosis of liver cells were more obvious.Conclusion In this study,the imaging indicators combined with blood and pathological indicators were used for multi-factor comprehensive evaluation of the modeling effect of cholecystitis and the stability of the inflammatory model,which was more intuitive,accurate and low-cost than the conventional gallbladder pathological analysis method or combined with enzyme-linked immunosorptive assay,and provided a scientific and effective detection index for the evaluation of therapeutic effects of drugs.At the same time,the model with the dose of 120mg/kg lincomycin hydrochloride for g

关 键 词：胆囊炎 豚鼠 模型优化 

分 类 号：R575[医药卫生—消化系统] R256.4[医药卫生—内科学]