朝那鸡MC1R基因G274A和A427G多态位点与羽色性状的关联分析  

Association of G274A and A427G polymorphisms of MC1R gene with feather color traits in Chaona chickens

作　　者：杨雪瑶 蔡翠翠 李玉莲 张恬[2] YANG Xueyao;CAI Cuicui;LI Yulian;ZHANG Tian(Guyuan Branch,NingXia Academy of Agriculture and Forestry Sciences,Guyuan 756000,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]宁夏农林科学院固原分院,宁夏固原756000 [2]中国中医科学院中药资源中心,北京100700

出　　处：《黑龙江畜牧兽医》2024年第2期60-64,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：宁夏回族自治区自然科学基金项目(2021AAC03288);宁夏回族自治区农业科技自主创新资金项目(DW-X-2020010)。

摘　　要：为了研究宁夏地方品种鸡朝那鸡的黑色素皮质激素1受体(melanocortin 1 receptor, MC1R)基因G274A和A427G多态位点与羽色性状的关联性,试验选择120日龄健康的朝那鸡150只(红羽、黑羽和白羽各50只)为试验对象,采用高分辨熔解曲线(high resolution melting, HRM)分析技术对朝那鸡MC1R基因G274A和A427G位点进行基因型分型检测,并且对基因型和羽色性状进行了关联分析。结果表明:试验朝那鸡群体MC1R基因G274A位点有GG、GA、AA三种基因型,其中AA基因型个体数量最多,等位基因A为优势等位基因,多态信息含量(PIC)为0.286 7,表现为中度多态(0.25≤PIC≤0.50),说明MC1R基因位点为G274A的朝那鸡群体选育潜力较高;A427G位点有AA、AG两种基因型,其中AA基因型个体数量最多,等位基因A为优势等位基因,PIC为0.062 3,表现为低度多态(PIC<0.25),说明MC1R基因位点为A427G的朝那鸡群体选育潜力较低;这两个位点均处于Hardy-Weinberg平衡状态(P>0.05)。G274A位点不同基因型间的羽色性状差异显著(P<0.05),黑羽群体中未发现GG基因型个体,红羽和白羽群体中GG基因型个体数量较GA、AA基因型个体少,AA基因型是红羽和黑羽群体中的优势基因型;A427G位点不同基因型间的羽色性状差异极显著(P<0.01),三个羽色群体中均未发现GG基因型个体,红羽群体均为AA基因型,黑羽和白羽群体中AA基因型个体数量较AG基因型多。说明MC1R基因这两个位点可作为朝那鸡羽色纯化的遗传标记应用于品种纯度鉴定和分子育种中。In order to study the association between G274A and A427G polymorphism of melanocortin 1 receptor(MC1R)gene and feather color traits in local Chaona chickens in Ningxia,150 healthy Chaona chickens(including 50 red feather,50 black feather and 50 white feather chickens)aged 120 days were selected as the experimental subjects.High resolution melting(HRM)analysis technology was used to detect the genotypes of C274A and A427G loci in Chaona chickens,and the association between genotypes and feather color traits was analyzed.The results showed that GG,GA and AA genotypes were found at the G274A locus of MCIR gene in the Chona chicken population.Among them,the number of AA genotype individuals was the largest,allele A was the dominant allele,'and PIC was O.2867,showing moderate polymorphism(0.25≤PIC≤0.50),indicating a high breeding potential in the Chona chicken population with MCIR gene locus G274A.Two genotypes,AAand AG,werefound at G274Alocus,among whichAAgenotype was the most abundant,lleleA was thedominant allele,and PIC was 0.0623,showing low polymorphism(PIC<0.25).These two sites were in Hardy-Weinberg equilibrium(P>0.05).There was a significant difference in feather color traits among different genotypes at C274A locus(P<0.05),there was no GG genotype in black feather population.The number of GG genotype individuals was less than that of GA and AA genotype individuals in red and white feather populations.The AA genotype was the dominant genotype in the red and black feather populations.There were very significant differences in feather color traits among different genotypes at A427G locus(P<0.01).CG genotypes were not found in the three feather populations,and AA genotype were found in the red feather populations.and the number of AA genotype individuals were more than that of AG genotype in black and white feather populations.The results indicated that the G274A and A427G polymorphic loci of MCIR gene could be used as genetic markers for the purification of feather color in breed purity identification and

关 键 词：地方鸡种 朝那鸡 MC1R基因 G274A位点 A427G位点 羽色性状 关联分析 

分 类 号：S831.2[农业科学—畜牧学]