蜂胶软胶囊中总黄酮测定方法的改进  

Improvement of the Determination Method for Total Flavonoids in Propolis Soft Capsules

作　　者：周璐 黄婷[1,2] 董耀 ZHOU Lu;HUANG Ting;DONG Yao(Jiangsu Provincial Productivity Promotion Center,Nanjing 210001,China;Jiangsu Provincial Physical and Chemical Testing Center,Nanjing 210001,China)

机构地区：[1]江苏省生产力促进中心,江苏南京210001 [2]江苏省理化测试中心,江苏南京210001

出　　处：《食品安全导刊》2024年第1期56-58,共3页China Food Safety Magazine

摘　　要：蜂胶中富含大量总黄酮类化合物,黄酮类化合物具有丰富的药理作用。对蜂胶软胶囊参照GB/T 20574—2006检测时,加入显色剂用水定容后,溶液变浑浊,影响分光光度计吸光度测定的稳定性,本文对其前处理条件、定容溶液、显色时间进行优化,并对方法标准曲线、重复性、加标回收率进行验证。结果表明,在415 nm处,以芦丁为标准品,在含量为0.2~1.2 mg时呈现良好的线性关系,线性回归方程为y=0.7341x-0.0056(R=0.9998),相对标准偏差为0.9%,加标回收率为92.0%~97.0%。Propolis is rich in a large amount of total flavonoids,which have rich pharmacological effects.When testing propolis soft capsules with reference to GB/T 20574—2006,the solution became turbid after adding a colorimetric agent to a constant volume of water,which affected the stability of the spectrophotometer absorbance measurement.The pre-treatment conditions,constant volume solution,and colorimetric time were improved,and the method standard curve,detection limit,repeatability,and spiked recovery rate were verified.The results showed that at 415 nm,the content of rutin was 0.2~1.2 mg,and the linear regression equation was y=0.7341x-0.0056(R=0.9998),the relative standard deviation was 0.9%.The recoveries were 92.0%~97.0%.

关 键 词：蜂胶软胶囊 总黄酮 分光光度法 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.3[轻工技术与工程—食品科学与工程]