高效液相色谱法测定莱芜鸡腿葱不同食用部位中维生素C含量的方法优化  

Optimization of High Performance Liquid Chromatography Method for Determining Vitamin C Content in Different Edible Parts of Laiwu Jitui Onion

作　　者：曹峰 高峰 王燕红 耿冬青 CAO Feng;GAO Feng;WANG Yanhong;GENG Dongqing(Jinan Food and Drug Inspection and Testing Center,Jinan 250102,China;Laiwu District Comprehensive Inspection and Testing Center,Jinan 271100,China)

机构地区：[1]济南市食品药品检验检测中心,山东济南250102 [2]莱芜区综合检验检测中心,山东济南271100

出　　处：《食品安全导刊》2024年第1期70-73,共4页China Food Safety Magazine

摘　　要：本文采用高效液相色谱法测定莱芜鸡腿葱葱茎及葱叶中的维生素C(L-抗坏血酸)含量,并进行了方法优化研究。样品采用1.0%的草酸溶液作为提取液超声提取2次,经Kromasil C_(18)柱分离后,用紫外检测器在254 nm波长处检测,以保留时间定性,外标法定量。结果显示,维生素C质量浓度在2.0~100.0 mg·L^(-1)时的线性关系较好(R=0.9995),精密度试验的相对标准偏差为1.33%(n=6),加标回收率为94.9%~96.1%,方法检出限为2.0×10^(-3)g·kg^(-1)。该测试方法简便快速,能准确测定莱芜鸡腿葱葱茎及葱叶中维生素C含量。In this paper,high performance liquid chromatography(HPLC)was used to determine the vitamin C(L-ascorbic acid)content in the onion stems and leaves of Laiwu Jitui onions,and the method was optimized.The samples were extracted twice with 1.0%oxalic acid solution as the extracting solution by ultrasonic extraction,separated on a Kromasil C_(18) column,and detected by an ultraviolet detector at 254 nm.The samples were qualitatively analyzed by the retention time,and quantitatively analyzed by the external standard method.The results showed that there was a good linear relationship(R=0.9995)when the mass concentration of vitamin C was between 2.0 mg·L^(-1) and 100.0 mg·L^(-1).The relative standard deviation of the precision test was 1.33%(n=6),the recovery rate was 94.9%~96.1%,and the detection limit of the method was 2.0×10^(-3)g·kg^(-1).This testing method is simple and fast,and can accurately determine the vitamin C content in the stems and leaves of Laiwu Jitui onion.

关 键 词：高效液相色谱法 莱芜鸡腿葱 葱茎 葱叶 维生素C 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]