橘红素经PI3K/Akt/mTOR信号通路调控胃癌细胞BGC-823和SGC-7901的增殖和凋亡  

Tangeretin regulates the proliferation and apoptosis of gastric cancer cells BGC-823 and SGC-7901 via PI3K/Akt/mTOR signaling pathway

作　　者：庄帅帅 郝润润 龚旖纯 黄磊 蒋魏 华杰 何晓璞 ZHUANG Shuaishuai;HAO Runrun;GONG Yichun;HUANG Lei;JIANG Wei;HUA Jie;HE Xiaopu(Department of Gastroenterology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210000;The First Clinical College of Nanjing Medical University;Department of Gastroenterology,Liyang People′s Hospital,China)

机构地区：[1]南京医科大学第一附属医院消化科,江苏南京210000 [2]南京医科大学第一临床医学院 [3]溧阳市人民医院消化内科

出　　处：《胃肠病学和肝病学杂志》2024年第2期162-166,170,共6页Chinese Journal of Gastroenterology and Hepatology

基　　金：溧阳市科技项目(LC2020002);江苏省干部保健科研课题(BJ19019)。

摘　　要：目的探讨橘红素对胃癌细胞BGC-823和SGC-7901的自噬作用及其机制。方法不同浓度橘红素(7.5、15、30、60、120μmol/L)处理两株细胞后,CCK-8法检测其吸光度值,计算细胞增殖抑制率;流式细胞仪检测细胞凋亡水平;用60μmol/L浓度的橘红素处理后通过细胞划痕法检测两种细胞迁移能力;Western blotting法检测自噬相关蛋白p62、凋亡蛋白Bax、Bcl-2及PI3K/Akt/mTOR信号通路相关蛋白表达的影响。结果与对照组相比,橘红素处理组增殖、迁移能力显著下降(P<0.01),细胞凋亡数增加,自噬蛋白p62的表达浓度依赖性升高(P<0.05),凋亡蛋白Bax表达增加(P<0.05),Bcl-2表达下降(P<0.05),磷酸化的p-Akt和p-mTOR的表达水平显著降低(P<0.05)。结论橘红素可通过抑制PI3K/Akt/mTOR信号通路调控SGC-7901和BGC-823细胞的增殖和凋亡,并可抑制其自噬。Objective To investigate the effect of Tangeretin on autophagy of gastric cancer cells BGC-823 and SGC-7901 and its mechanism.Methods After treating the two kinds of cells with different concentrations of Tangeretin(7.5,15,30,60,120μmol/L),the absorbance value was detected by CCK-8 method to calculate the cell proliferation inhibition rate.Flow cytometry was used to detect cell apoptosis level;treated with Tangeretin at a concentration of 60μmol/L to detect the migration ability of the two cells by cell scratching method.Western blotting method was used to detect autophagy-related protein p62,apoptosis protein Bax,Bcl-2 and PI3K/Akt/mTOR signaling pathway.The influence of pathway related protein expression.Results Compared with the control group,the proliferation and migration ability of the Tangerine group was significantly decreased(P<0.01).The number of apoptosis increased,and the expression of autophagy protein p62 increased in a concentration-dependent manner(P<0.05).The expression of apoptosis protein Bax increased(P<0.05),Bcl-2 expression decreased(P<0.05),and the expression levels of phosphorylated p-Akt and p-mTOR decreased significantly(P<0.05).Conclusion Tangeretin can regulate the proliferation,apoptosis and migration of SGC-7901 and BGC-823 gastric cancer cells by inhibiting the PI3K/Akt/mTOR signaling pathway,and can inhibit autophagy.

关 键 词：橘红素 胃癌 自噬 增殖 凋亡 PI3K/Akt/mTOR信号通路 

分 类 号：R735.2[医药卫生—肿瘤]