SOCS-3蛋白及其基因多态性对肥胖胃食管反流病患者食管黏膜损伤的影响  

Effect of SOCS-3 and its gene polymorphism on esophageal mucosal injury in obese patients with gastroesophageal reflux disease

作　　者：申海剑 年媛媛 孟宪梅[2] SHEN Haijian;NIAN Yuanyuan;MENG Xianmei(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040;Department of Gastroenterology,the Second Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,China)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]包头医学院第二附属医院消化内科

出　　处：《胃肠病学和肝病学杂志》2024年第2期167-170,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：内蒙古自治区自然科学基金项目(2019LH08042);内蒙古自治区科技创新引导项目。

摘　　要：目的探讨SOCS-3蛋白及其基因多态性对肥胖人群胃食管反流病(gastroesophageal reflux disease,GERD)食管黏膜损伤的影响。方法选取100例GerdQ量表评分>8分的GERD患者。采用Western blotting方法研究SOCS-3的蛋白表达情况。采用PCR-测序法对SOCS-3基因rs4969168位点进行基因分型,并分析肥胖相关指标(BMI、体质量、身高、腰围)、反流性食管炎(reflux esophagitis,RE)洛杉矶分级与rs4969168位点基因多态性的相关性。结果肥胖组GERD患者SOCS-3蛋白表达最高,超重组其次,正常组最低。不同基因型GERD患者BMI值:AA(26.21±3.41)kg/m^(2)、AG(24.42±2.04)kg/m^(2)、GG(23.90±2.68)kg/m^(2)(P=0.011),腰围值:AA(91.34±10.85)cm、AG(88.90±8.93)cm、GG(84.28±8.00)cm(P=0.03),rs4969168位点基因型中等位基因A出现频率与BMI、腰围的平均水平呈正相关。GERD患者食管黏膜损伤程度与rs4969168基因多态性显著相关(P=0.037);随着A等位基因出现频率减少,GERD患者出现严重食管黏膜损伤的比例增加。结论rs4969168的等位基因A在GERD人群食管黏膜炎症损伤中起保护作用;对于高BMI的GERD患者,食管黏膜SOCS-3蛋白表达增高可能是等位基因A产生保护作用的重要因素。Objective To investigate the effect of SOCS-3 gene polymorphism on esophageal mucosal injury in obese people gastroesophageal reflux disease(GERD).Methods A total of 100 patients with GERD with more than 8 scores of Gerd Q scale were randomly selected.Based on PCR-sequencing,the genotyping of SNP polymorphic rs4969168 in SOCS-3 gene was studied.The correlation between obesity-related indexes(BMI,weight,height,waist circumference),GERD Los Angeles grade and its gene polymorphism were studied,and the expression of SOCS-3 was studied by Western blotting.Results The expression of SOCS-3 protein in obese GERD patients was the highest,the overweight group was the second,and the normal group was the lowest.The value of BMI in patients with different genotypes of GERD,AA:(26.21±3.41)kg/m^(2),AG:(24.42±2.04)kg/m^(2),GG:(23.90±2.68)kg/m^(2)(P=0.011),and the waist circumference of patients with different genotypes of GERD,AA:(91.34±10.85)cm,AG:(88.90±8.93)cm,GG:(84.28±8.00)cm(P=0.03).The frequency of allele A at rs4969168 locus was positively correlated with the average level of BMI and waist circumference.The degree of esophageal mucosal injury in GERD patients was significantly associated with rs4969168 gene polymorphism(P=0.037).With the decrease of the frequency of allele A,the proportion of severe esophageal mucosal injury in GERD patients increased.Conclusion Allele A of rs4969168 plays a protective role in esophageal mucosal inflammatory injury in patients with GERD,and the increased expression of SOCS-3 protein in esophageal mucosa may be an important factor in the protective effect of allele A in patients with high BMI.

关 键 词：肥胖 胃食管反流病 SOCS-3 基因多态性 

分 类 号：R571[医药卫生—消化系统]