外源ABA处理对红阳猕猴桃果实采后成熟及关键基因的影响  

Effects of ABA Treatment on Postharvest Ripening and Key Genes of Actinidia chinensis cv.‘Hongyang’

作　　者：兰德国 廖星强 翟夏琬 贾茗淑 赵连鑫 杨清群 陈金印 开文斌 LAN Deguo;LIAO Xingqiang;ZHAI Xiawan;JIA Mingshu;ZHAO Lianxin;YANG Yingqun;CHEN Jinyin;KAI Wenbin(School of Agricultural Sciences,Jiangxi Agricultural University/Collaborative Innovation Center ofPostharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province/Jiangxi KeyLaboratory for Postharvest Technology and Non-destructive Testing of Fruits&Vegetables,Nanchang 330045,China;Wuwei Academy of Forestry,Wuwei,Gansu 733000,China;Jiujiang Forestry Science ResearchInstitute,Jiujiang,Jiangxi 332006,China)

机构地区：[1]江西农业大学农学院/江西省果蔬保鲜与无损检测重点实验室/江西省果蔬采后处理关键技术与质量安全协同创新中心,江西南昌330045 [2]武威市林业科学研究院,甘肃武威733000 [3]九江市林业科学研究所,江西九江332006

出　　处：《江西农业大学学报》2023年第6期1358-1369,共12页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(32260780);中国博士后科学基金项目(2021M701513)。

摘　　要：【目的】探究脱落酸(Abscisic acid,ABA)处理对猕猴桃采后果实成熟及果实品质的影响,为精准调控猕猴桃果实采后成熟过程,延长贮藏期提供理论依据和参考。【方法】以‘红阳’猕猴桃为试材,在采收后分别使用100µmol/L ABA和200µmol/L去甲二氢愈创木酸(NDGA,ABA合成抑制剂)进行浸果处理,以清水处理为对照。测定采后果实呼吸强度、失重率、硬度、可溶性固形物、可滴定酸、维生素C和可溶性糖含量,探究ABA处理对猕猴桃果实贮藏品质的影响。检测内源ABA含量和乙烯释放量,并利用qRT-PCR方法分析ABA代谢关键基因(AcNCEDs和AcCYP707As)和ABA信号核心组分基因(AcPYLs、AcPP2Cs和AcSnRK2s)的表达,以及乙烯相关和细胞壁降解相关基因的转录表达差异。【结果】猕猴桃果实在常温贮藏期内,果实硬度逐渐下降,ABA处理组均低于对照组,同时在贮藏第8天时,ABA显著提高了细胞壁降解相关基因AcPL1、AcPL2、AcXTH5、AcXTH6、AcPME1和AcMAN1的表达量,分别为对照的3.01、2.42、7.58、21.47、5.11和9.49倍,表明ABA处理可能通过诱导细胞壁降解相关基因的表达来促进果实软化。外源ABA能促进猕猴桃内源ABA的生物合成和乙烯的释放,AcNCED1、AcNCED2的表达模式与ABA含量的趋势相一致,同时对乙烯相关基因的表达分析发现,ABA处理促进了乙烯合成和信号转导基因的上调。此外,ABA处理能促进可滴定酸和维生素C的降解,促进可溶性固形物、总糖和可溶性糖的积累,提高了果实呼吸速率和失重率。这些结果表明ABA促进猕猴桃采后成熟,并影响了果实品质。【结论】外源ABA处理促进了猕猴桃果实的采后成熟,激发了乙烯合成和信号相关基因,以及细胞壁降解相关基因的表达,加速了果实软化。研究结果为揭示ABA促进猕猴桃采后成熟的分子机制提供了一定的理论依据。[Objective]The aim of this study was to investigate the effects of abscisic acid(ABA)treatment on postharvest fruit ripening and fruit quality of kiwifruit,thus providing theoretical basis and reference for precise regulation of postharvest ripening process and prolonging storage period of kiwifruit.[Method]In present study,‘Hongyang’was used as the experimental material.The kiwifruit clusters were treated with 100µmol/L ABA and 200µmol/L nordihydroguaiaretic acid(NDGA,an inhibitor of ABA synthesis)for fruit soaking after harvesting,and water treatment was used as the control.Postharvest fruit respiration intensity,weight loss,hardness,soluble solids,titratable acid,vitamin C and soluble sugar contents were measured to investigate the effects of ABA treatment on the storage quality of kiwifruit fruit.Endogenous ABA content and ethylene release were detected,the expression of key genes of ABA metabolism(AcNCEDs and AcCYP707As)and core component genes of ABA signaling(AcPYLs,AcPP2Cs and AcSnRK2s),as well as the transcriptional expression differences of ethylene-related and cell wall degradation-related genes were analyzed by qRT-PCR.[Result]During the storage period at room temperature,fruit hardness of kiwifruit fruits gradually decreased,and the hardness of all ABA-treated groups were lower than that of the control group,while at the 8th day of storage,ABA significantly increased the expression of cell wall degradation-related genes AcPL1,AcPL2,AcXTH5,AcXTH6,AcPME1 and AcMAN1,which were 3.01,2.42,7.58,21.47,5.11 and 9.49-fold,respectively,indicating that ABA treatment may promote fruit softening by inducing the expression of genes related to cell wall degradation.Exogenous ABA promoted endogenous ABA biosynthesis and ethylene release in kiwifruit.Tthe expression patterns of AcNCED1 and AcNCED2 were consistent with the trend of ABA content,while the expression analysis of ethylene-related genes revealed that ABA treatment promoted the upregulation of ethylene synthesis and signal transduction genes.In additi

关 键 词：ABA 猕猴桃 采后成熟 基因表达 

分 类 号：S663.4[农业科学—果树学]