中性蛋白酶高温酶解条件下猪粪资源化利用中养分的变化规律  

Changes Rule of Nutrients During the Resource Utilization of Pig Manure Under High Temperature Enzymatic Hydrolysis with Neutral Protease

作　　者：戴鹏飞 孙权[1] 解昊郡 王锐[1] 蒋鹏[1] DAI Peng-fei;SUN Quan;XIE Hao-jun(College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《安徽农业科学》2024年第4期51-56,共6页Journal of Anhui Agricultural Sciences

基　　金：宁夏回族自治区重点研发计划项目(2019BCF01001,2022BEG02004)。

摘　　要：以酶工程及酶动力学为技术依托及科学依据,将猪粪加玉米秸秆组合作为底物,添加2%中性蛋白酶,在生化反应釜中80℃酶解3 h,底物含水量降低至56.01%,同比下降2.34%;大分子有机质含量降低至63.79%,酶解后,A、B处理的底物有机质含量分别降低了14.19%、15.06%;植物可利用大量元素提高至6.87%,符合NY 525—2012有机肥标准;速效磷、速效钾、全盐、总腐殖酸呈下降趋势;蛔虫卵死亡率达到92%,种子发芽指数达到54%,达到有机肥腐熟标准。Based on enzyme engineering and enzyme kinetics as the technical support and scientific basis,the combination of pig manure and corn stover was used as the substrate,2%neutral protease was added,and the water content of the substrate was reduced to 56.01%at 80°C for 3 h in a biochemical reactor,a year-on-year decrease of 2.34%.The content of macromolecular organic matter was reduced to 63.79%,and after enzymatic hydrolysis,the organic content of substrates treated A and B reached 14.19%and 15.06%,respectively.The use of a large number of elements in plants had been increased to 6.87%,which met the NY 525-2012 organic fertilizer standard.Available-available phosphorus,available potassium,total salt and total humic acid showed a downward trend.The mortality rate of roundworm eggs reached 92%,and the seed germination index reached 54%,which reached the standard of organic fertilizer rot.

关 键 词：猪粪 中性蛋白酶 高温酶解 有机肥 养分 变化规律 

分 类 号：S141.2[农业科学—肥料学]