可降解PLA/PBAT塑料的微生物菌群结构研究  

Study on the Microbial Community Structure of Degradable PLA/PBAT Composites

作　　者：裴雯玉 江雨韩 李娜[1] 陈怡 吴弘 PEI Wen-yu;JIANG Yu-han;LI Na(College of Life Sciences,Tianjin Normal University,Tianjin 300387)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《安徽农业科学》2024年第4期57-61,70,共6页Journal of Anhui Agricultural Sciences

基　　金：天津市科学计划项目(18ZLZXZF00220);天津市级“大学生创新创业训练计划”(202210065167);天津师范大学引进人才基金项目(5RL147)。

摘　　要：为探究PLA/PBAT复合材料在菌群处理下的降解情况,加快该塑料的生物降解速率。通过富集培养法筛选获得不同批次的可降解菌群,基于失重法评估不同批次菌群对于PLA/PBAT复合材料的降解能力,并利用高通量测序分析批次间微生物的结构特点。结果发现,通过多轮富集后,在相同的处理周期内,PLA/PBAT塑料的失重率由2.52%提高到4.93%。转接多批次后在门分类水平上菌群丰度最高的5个菌门分别为拟杆菌门、浮霉菌门、变形菌门、装甲菌门和绿弯菌门,占比超过95.00%;在属水平上,丰度较高的分别为拟杆菌门OPB56属和浮霉菌门SH-PL14属。微生物群落结构随着不断的转接而趋于稳定。In order to explore the degradation of PLA/PBAT composites under the treatment of bacteria,we analyzed the characteristics of microbiome and accelerated the biodegradation rate.In this study,different batches of bacteria were screened and their degradation ability were evaluated based on weightlessness method.The characteristics of microorganisms among different batches were analyzed by high-throughput sequencing.The results showed that the weight loss of PLA/PBAT plastics increased from 2.52%to 4.93%.The five highest abundance at phyla level were Bacteroidetes,Planctomycetes,Proteobacteria,Armatimonadetes and Chloroflexi,accounting for more than 95.00%.OPB56 and SH-PL14 were the highest abundance at genus level.The microbial community structure tended to be stable.

关 键 词：PLA/PBAT塑料 失重率 高通量测序 菌群结构 

分 类 号：X172[环境科学与工程—环境科学]