食品安全检测仪快速测定板栗中脱氢乙酸含量  

Rapid Determination of Dehydroacetic Acid Content in Chestnuts Using Food Safety Detector

作　　者：徐加庆 董晓毅 李倩 姚思坤 江方雷 XU Jia-qing;DONG Xiao-yi;LI Qian(Xiamen Vine Biotechnology Co.,Ltd.,Xiamen,Fujian 361021;Southeast Fujian Geology Brigade,Quanzhou,Fujian 362011)

机构地区：[1]厦门市藤蔓生物科技有限公司,福建厦门361021 [2]福建省闽东南地质大队,福建泉州362011

出　　处：《安徽农业科学》2024年第4期170-172,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立食品安全检测仪快速测定板栗中脱氢乙酸(DHA)含量的分析方法。[方法]用410 nm波长的食品安全检测仪测定标准溶液浓度并绘制标准曲线。经过丙酮、甲醇萃取后的板栗与检测试剂反应,将反应液放入仪器中自动计算并得出脱氧乙酸浓度。[结果]被测物中脱氢乙酸均能得到很好的提取分离,空白溶剂无干扰;标准溶液浓度在0~2.5 g/kg线性关系良好(R^(2)=0.9998),方法检出限和定量限分别为0.0014和0.0044 g/kg;6种加工板栗样品中脱氢乙酸含量分别为0.156、0.321、0.254、0.450、0.285和0.389 g/kg,相对标准偏差(RSD)在1.5%~2.4%;空白样品在0.2、0.6、1.0 g/kg 3种不同加标量下平均回收率在100.1%~101.7%,RSD在0.9%~2.9%;该方法与国标法对比的测试结果无显著差异。[结论]该方法具有提取过程简便、操作简单、用时短、性价比高、回收率较高、结果准确可靠且重现性较好等优点,适合批量样品快速初筛检测。[Objective]To establish an analytical method for rapid determination of dehydroacetic acid(DHA)content in chestnuts using food safety detector.[Method]A food safety detector with a wavelength of 410 nm was used to measure the concentration of the standard solution and draw a standard curve.After extraction with acetone and methanol,the chestnuts reacted with detection reagents,and the reaction solution was automatically calculated and the concentration of deoxyacetic acid was obtained by putting it into the instrument.[Result]Dehydroacetic acid in the tested substance could be well extracted and separated,and the blank solvent had no interference;the standard solution concentration had a good linear relationship between 0-2.5 g/kg(R^(2)=0.9998),the detection limit and quantification limit of the method were 0.0014 and 0.0044 g/kg,respectively.The dehydroacetic acid content in six processed chestnut samples was 0.156,0.321,0.254,0.450,0.285 and 0.389 g/kg,respectively,with relative standard deviation(RSD)ranging from 1.5%to 2.4%.The average recovery rate of blank samples at three different spiking amounts of 0.2,0.6 and 1.0 g/kg ranged from 100.1%to 101.7%,with RSD ranging from 0.9%to 2.9%;there was no significant difference in the test results between this method and the national standard method.[Conclusion]This method has the advantages of simple extraction process,simple operation,short time,high cost-effectiveness,high recovery rate,accurate and reliable results,and good reproducibility,making it suitable for rapid primary screening detection of batch samples.

关 键 词：板栗 脱氢乙酸 食品安全检测仪 快速检测 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]