多级双水相萃取木瓜蛋白酶的研究  

Study on multi-stage aqueous two-phase extraction of papain

作　　者：朱恒伟 白新鹏[1,2,3] 梁秋杨 钟泷彬 常会敏 陈运坤 乔振栓 ZHU Hengwei;BAI Xinpeng;LIANG Qiuyang;ZHONG Longbin;CHANG Huimin;CHEN Yunkun;QIAO Zhenshuan(College of Food Science and Technology,Hainan University,Haikou 570203,China;Engineering Research Center of Utilization of Tropical Polysaccharide Resources,Ministry of Education,Haikou 570203,China;Haikou Zhisu Biological Resources Research Institute Co.Ltd.,Haikou 570100,China)

机构地区：[1]海南大学食品科学与工程学院,海南海口570203 [2]热带多糖资源利用教育部工程研究中心,海南海口570203 [3]海口植之素生物资源研究所有限公司,海南海口570100

出　　处：《食品与发酵工业》2024年第4期152-156,共5页Food and Fermentation Industries

基　　金：海南省重点科技计划项目(ZDYF2022XDNY146)。

摘　　要：使用双水相萃取大规模生产木瓜蛋白酶时,为获得较高的回收率,通常需要较高的萃取剂浓度和体积比,但这会导致目标物的纯度和浓度降低。该研究采用聚乙二醇和柠檬酸钠组成的双水相体系在多个离心管之间的转移来模拟工业上常用的多级错流萃取和多级逆流萃取过程,以分配系数、酶活性回收率为指标,采用McCabe Thiele图解法得到多级逆流双水相萃取的理论级数,结果表明,当相比为0.5,原料液下相的木瓜蛋白酶的初始质量浓度为0.20 mg/mL时,使用3级逆流双水相萃取木瓜蛋白酶的酶活性回收率达到了96.75%,同时设计出多级逆流双水相萃取木瓜蛋白酶的优化方案。For large-scale production of papain using aqueous two-phase extraction(ATPE),high extractant concentrations and volume ratio are usually required to obtain high recovery,but this results in a decrease in the purity and concentration of the target.In this study,the aqueous two-phase system composed of polyethylene glycol(PEG)and sodium citrate(Na 3C 6H 5O 7)was transferred between multiple centrifuge tubes to simulate the multi-stage crosscurrent extraction and multi-stage countercurrent extraction processes commonly used in industry,and the theoretical number of stages of multi-stage countercurrent ATPE was obtained by McCabe Thiele diagram method with the indexes of partition coefficient and recovery of enzyme activity.The results showed that the recovery of papain activity using the three-stage countercurrent ATPE achieved 96.75%when the volume radio was 0.5 and the initial concentration of papain in the bottom phase of the feedstock was 0.20 mg/mL,while an optimized scheme for multi-stage countercurrent ATPE of papain was designed.

关 键 词：木瓜蛋白酶 双水相体系 多级错流萃取 多级逆流萃取 McCabe Thiele图解法 

分 类 号：TQ936[化学工程]